Dissection of the region of Pseudomonas aeruginosa ParA that is important for dimerization and interactions with its partner ParB.
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Purified ParA hydrolyses ATP.
"ParA-His6 demonstrated ATPase activity (Fig. 3) of ~1.2 pmol Pi released min−1 (pmol ParA)−1."
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ParA dimerization is needed for interaction with ParB.
"interactions depend on the ability of ParA to dimerize."
Deletion of the parA (soj) homologue in Pseudomonas aeruginosa causes ParB instability and affects growth rate, chromosome segregation, and motility.
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parA loss or excess causes anucleate cells and motility changes.
"Both inactivation of parA and overexpression of parA in trans in P. aeruginosa also lead to accumulation of anucleate cells and changes in motility."
ParB deficiency in Pseudomonas aeruginosa destabilizes the partner protein ParA and affects a variety of physiological parameters.
A single parS sequence from the cluster of four sites closest to oriC is necessary and sufficient for proper chromosome segregation in Pseudomonas aeruginosa.
Soj (ParA) DNA binding is mediated by conserved arginines and is essential for plasmid segregation.
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The Soj DNA-binding arginines are conserved in P. aeruginosa ParA.
"The arginine residues are conserved among other chromosomal homologues, including the ParAs from Caulobacter crescentus, Pseudomonas aeruginosa, Pseudomonas putida"
Molecular basis of ParA ATPase activation by the CTPase ParB during bacterial chromosome segregation.
Pairwise comparison of P. putida and P. aeruginosa ParA and ParB