Use of the ND evidence code for Gene Ontology (GO) terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
UniProtKB entry for GTT3_YEAST (P39996)
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Gtt3 is a multi-pass membrane protein with two transmembrane helices and is localized to the nuclear membrane; it is named glutathione transferase 3 but no molecular function is experimentally established.
"SUBCELLULAR LOCATION: Nucleus membrane {ECO:0000269|PubMed:14562095};"
Global analysis of protein localization in budding yeast.
One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy.
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SWAp-Tag N'-terminal GFP library methods paper; source of the SGD HDA nuclear-periphery localization for Gtt3. Localization categories assigned by manual imaging include "Nuclear periphery".
"Nuclear periphery; Nucleolus; Nucleus"
Falcon deep research report for GTT3 (YEL017W)
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Comprehensive literature synthesis finding no direct biochemical, enzymatic, or localization characterization specific to Gtt3, and noting its distinct domain architecture and lack of oxidative-stress co-regulation with Gtt1/Gtt2.
"no direct enzymatic assay, substrate specificity, or catalytic mechanism for the specific YEL017W protein was retrieved"