Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
Cloning and characterization of Xenopus Id4 reveals differing roles for Id genes.
-
Id3 expression is induced by BMP4 and Notch; in animal caps Id3 selectively inhibits NeuroD activity.
"Id4 is able to inhibit the activity all these bHLH molecules, Id2 inhibits MyoD and neuroD, while Id3 blocks only neuroD activity in our assays."
Id expression in amphioxus and lamprey highlights the role of gene cooption during neural crest evolution.
-
Id expression at the neural plate border and dorsal neural tube is a vertebrate novelty already present in lamprey; mesoderm and endoderm expression is conserved with amphioxus.
"while expression of Id genes in the mesoderm and endoderm is conserved between amphioxus and vertebrates, expression in the lateral neural plate border and dorsal neural tube is a vertebrate novelty"
To proliferate or to die: role of Id3 in cell cycle progression and survival of neural crest progenitors.
-
Id3 depletion causes cell-cycle arrest and death of neural crest progenitors rather than a fate switch; overexpression expands the neural crest.
"This appears to be mediated by cell cycle inhibition followed by cell death of the neural crest progenitor pool, rather than a cell fate switch."
Xenopus Id3 is required downstream of Myc for the formation of multipotent neural crest progenitor cells.
-
Id3 is a Myc target required for formation and maintenance of neural crest stem cells; forced expression maintains the progenitor state.
"the small HLH protein Id3 is a Myc target that plays an essential role in the formation and maintenance of neural crest stem cells"
Hairy2 functions through both DNA-binding and non DNA-binding mechanisms at the neural plate border in Xenopus.
Hairy2-Id3 interactions play an essential role in Xenopus neural crest progenitor specification.
-
Id3 physically interacts with Hairy2 and negatively regulates its activity, yet is required for Hairy2 to promote neural crest formation.
"However, Id3 is required to allow Hairy2 to promote neural crest formation."
Self-regulation of Stat3 activity coordinates cell-cycle progression and neural crest specification.
-
Id3 disrupts Stat3-FGFR4 complex formation, providing negative feedback on FGF-driven Stat3 activation in neural crest progenitors.
"Stat3 activation is also modulated by Hairy2 and Id3 proteins that, respectively, facilitate and disrupt Stat3-FGFR4 complex formation."
NEURODEVELOPMENT. Shared regulatory programs suggest retention of blastula-stage potential in neural crest cells.
-
Id3 is co-expressed with core pluripotency factors in Xenopus blastula cells and is a Myc target required both for neural crest genesis and ES cell pluripotency.
"This model is supported by a shared requirement for Myc protein, and its transcriptional target Id3, in both neural crest cell genesis and ES cell pluripotency"
Id gene activity during Xenopus embryogenesis.
XIdx, a dominant negative regulator of bHLH function in early Xenopus embryos.
-
XIdx (Id3) disrupts myogenic factor/E-protein DNA binding and inhibits MyoD transactivation in embryonic tissue.
"disrupts binding of myogenic factor/E-protein complexes to DNA in vitro and inhibits transactivation of the E-box regulated cardiac actin gene by MyoD in embryonic tissue"
Falcon deep research report for Xenopus laevis id3-a