Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Combined Automated Annotation using Multiple IEA Methods
The Fanconi anemia protein FANCF forms a nuclear complex with FANCA, FANCC and FANCG.
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FANCF is predominantly nuclear and forms a complex with FANCA, FANCC and FANCG; these interactions are lost in FA cell lines, indicating each subunit is required for complex formation.
"FANCF was found predominantly in the nucleus, where it complexes with FANCA, FANCC and FANCG."
Fanconi anemia protein complex: mapping protein interactions in the yeast 2- and 3-hybrid systems.
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FANCG binds both the N-terminus of FANCA and the C-terminal region of FANCF, and can bridge FANCA to FANCF, mapping the physical architecture underlying FANCF's adaptor role.
"FANCG was shown to interact with both the amino-terminus of FANCA and the carboxyl-terminal region of FANCF."
The Fanconi anemia gene product FANCF is a flexible adaptor protein.
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FANCF is a flexible adaptor whose C-terminus binds FANCG to nucleate assembly of the other FA proteins, while its N-terminus stabilizes the FANCA/FANCG interaction and is required to recruit the FANCC/FANCE subcomplex; FANCF does not have a ROM-like function.
"We found that the C terminus of FANCF interacts directly with FANCG and allows the assembly of other FA proteins into a stable complex."
Structural determinants of human FANCF protein that function in the assembly of a DNA damage signaling complex.
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The FANCF C-terminal domain has a Cand1-like helical-repeat fold and functions as a molecular adaptor bridging the A:G and C:E subcomplexes; two C-terminal loops are essential for FANCD2 monoubiquitination and mitomycin C resistance.
"The human FANCF protein reportedly functions as a molecular adaptor within the FA nuclear complex, bridging between the subcomplexes A:G and C:E."
The Fanconi anemia pathway promotes replication-dependent DNA interstrand cross-link repair.
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FANCI-FANCD2 (activated by the FA core complex) is required for replication-coupled ICL repair in S phase, including incisions and translesion synthesis.
"FANCI-FANCD2 is required for replication-coupled ICL repair in S phase."
A histone-fold complex and FANCM form a conserved DNA-remodeling complex to maintain genome stability.
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FANCM-MHF associates with the FA core complex and promotes FANCD2 monoubiquitination in response to DNA damage.
"FANCM-MHF associates with the Fanconi anemia (FA) core complex, promotes FANCD2 monoubiquitination in response to DNA damage"
Fancf-deficient mice are prone to develop ovarian tumours.
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Fancf-deficient cells show an aberrant response to DNA cross-linking agents (G2 arrest, chromosomal aberrations, reduced survival, inability to monoubiquitinate FANCD2), providing in vivo confirmation that FANCF is required for FANCD2 activation and the DNA cross-link damage response.
"they showed an aberrant response to DNA cross-linking agents as manifested by G(2) arrest, chromosomal aberrations, reduced survival, and an inability to monoubiquitinate FANCD2."
FAAP20: a novel ubiquitin-binding FA nuclear core-complex protein required for functional integrity of the FA-BRCA DNA repair pathway.
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FAAP20 is an integral FA core-complex protein whose UBZ domain is required for DNA-damage-induced chromatin loading of FANCA and functional integrity of the FA pathway.
"is required for DNA-damage-induced chromatin loading of FANCA and the functional integrity of the FA pathway"
A reference map of the human binary protein interactome.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
FA core complex assembles at DNA interstrand crosslinks (ICLs)
FANCD2:FANCI complex and UBE2T bind ICL-DNA associated with the FA core complex
Monoubiquitination of FANCD2:FANCI
DNA nucleases bind monoubiquitinated ID2 complex
DNA nucleases unhook the interstrand crosslink (ICL)
Translesion synthesis across unhooked ICL by POLN
FANCD2 deubiquitination by USP1:WDR48
The complex of ATR and ATRIP is recruited to ICL-DNA
ATR phosphorylates RPA2, FANCI, FANCD2 and FANCM at ICL-DNA
FA core complex:HSP70s binds PKR