Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
The cyclic nucleotide specificity of three cAMP receptors in Dictyostelium.
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cAR2 was expressed and assayed by [3H]cAMP binding; unlike cAR1 and cAR3 it showed no detectable affinity in phosphate buffer and only an 11 nM affinity state in ammonium sulfate, marking cAR2 as a comparatively low/atypical-affinity cAMP receptor.
"In phosphate buffer, there were two affinity states of about 30 and 300 nM for cAR1 and 20 and 500 nM for cAR3 but no detectable affinity for cAR2."
Chemoattractant signaling in dictyostelium discoideum.
The cyclic nucleotide specificity of eight cAMP-binding proteins in Dictyostelium discoideum is correlated into three groups.
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The four cell-surface cAMP receptors (including cAR2) form one pharmacological group and bind cAMP to transduce the signal during chemotaxis and cell differentiation.
"the cell surface receptors participate in the transduction of the cAMP signal during chemotaxis and cell differentiation"
Transcriptomic and metabolomic insights into light-mediated unicellular-to-multicellular transition in Dictyostelium discoideum.
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carB (CarB) is among genes down-regulated under dark relative to light during the unicellular-to-multicellular transition, linking cAR2 expression to light-modulated development.
"some downregulated genes including adenylate cyclase (acrA) and cyclic AMP receptor (CarB) could reduce the cell aggregation during dark incubation"
CAR2, a prestalk cAMP receptor required for normal tip formation and late development of Dictyostelium discoideum.
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cAR2 is a G protein-linked, seven-transmembrane cell-surface receptor for cAMP.
"The receptors for cAMP are members of the family of cell-surface receptors that are linked to G proteins and characterized by seven putative transmembrane domains."
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cAR2 is expressed only after aggregation and preferentially in prestalk cells.
"CAR2 is expressed only after cells have aggregated and, then, preferentially in prestalk cells."
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cAR2-null cells arrest at the tight-mound stage and over-express prespore markers, and cAR2 is required for cAMP-directed prestalk sorting.
"CAR2 may be required for cAMP-directed sorting of prestalk cells during pattern formation within the aggregation mound."
Switching of chemoattractant receptors programs development and morphogenesis in Dictyostelium: receptor subtypes activate common responses at different agonist concentrations.
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cAR2, when expressed, can mediate chemotaxis, adenylyl cyclase activation and actin polymerization, and cAR subtypes activate common responses at different cAMP concentrations.
"assessed the capacity of each to mediate chemotaxis, activation of adenylyl cyclase and actin polymerization"