Gene Ontology annotation through association of InterPro records with GO terms

Electronic Gene Ontology annotations created by ARBA machine learning models

Combined Automated Annotation using Multiple IEA Methods

PMID:20158506

Identification of conditionally essential genes for growth of Pseudomonas putida KT2440 on minimal medium through the screening of a genome-wide mutant library

In P. putida KT2440 the histidine-biosynthesis genes PP0965-PP0967 are annotated as the hisGDC cluster, with PP_0967 corresponding to hisC, and RT-PCR co-transcription assays support operon organization of these clusters.

PMID:11518529

Crystal structure of histidinol phosphate aminotransferase (HisC) from Escherichia coli, and its covalent complex with pyridoxal-5'-phosphate and L-histidinol phosphate

E. coli HisC is a PLP-dependent homodimeric aminotransferase with active sites at the dimer interface; the active-site lysine (Lys214) forms an internal aldimine with PLP and catalysis proceeds through PMP via a ping-pong mechanism.

file:PSEPK/hisC/hisC-deep-research-openscientist.md

OpenScientist functional report for PSEPK HisC

Supports the exact HisC reaction and histidine-pathway assignment for Q88P86 while distinguishing family-level aromatic-amino-acid activity from a demonstrated physiological role in KT2440.