Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
The carboxyl methyltransferase modifying G proteins is a metalloenzyme.
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PPMT/ICMT catalyzes posttranslational methylation of isoprenylated C-terminal cysteine residues of small monomeric G proteins (Ras, Cdc42, RhoA, Rab1) and heterotrimeric G-protein gamma subunits, and is a metalloenzyme requiring divalent cations.
"posttranslational methylation of isoprenylated C-terminal cysteine residues"
Topology of mammalian isoprenylcysteine carboxyl methyltransferase determined in live cells with a fluorescent probe.
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Human ICMT is an integral ER membrane protein that traverses the ER membrane eight times with both termini cytosolic; methyl esterification neutralizes prenylcysteine charge and increases membrane affinity.
"integral membrane protein restricted to the endoplasmic reticulum"
Regulation of NOTCH signaling by RAB7 and RAB8 requires carboxyl methylation by ICMT.
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ICMT methylesterifies C-terminal prenylcysteine residues of CaaX proteins and some RAB GTPases (RAB7, RAB8); methylation is required for their endomembrane localization, GTP loading, and downstream NOTCH signaling.
"Isoprenylcysteine carboxyl methyltransferase (ICMT) methylesterifies C-terminal"
A reference map of the human binary protein interactome.
Mammalian prenylcysteine carboxyl methyltransferase is in the endoplasmic reticulum.
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pcCMT/ICMT is the third of three enzymes that modify C-terminal CAAX motifs; the mammalian enzyme is a multi-pass membrane protein restricted to the ER, not the plasma membrane.
"Mammalian pcCMT was not expressed at the plasma membrane"
ICMT:Zn2+ transfers CH3 from AdoMet to isoprenylated proteins
ICMT methylates S-Farn RAS proteins
Cysmethynil binds ICMT:Zn2+