rad50 (Drosophila RAD50) review notes

Literature journal

• Null phenotypes: PMID:15296753; localization PMID:15296753; PMID:15296753.
• Capping: PMID:15296753
• P-element allele: PMID:15135728
• No telomerase in Drosophila: PMID:15296751.

Curation decisions

• Core: ATPase subunit of MRN in DSB processing/repair; telomere capping.
• Telomere maintenance via telomerase (IBA) removed: no telomerase in Drosophila.
• Telomere maintenance via recombination: IBA, IEA and IMP rows all modified to telomere capping (GO:0016233), a sibling term under telomere maintenance (GO:0000723). The IBA/IEA rows were initially marked over-annotated; they were aligned with the IMP row in PR review follow-up because the argument (fly telomeres are capped, not lengthened by recombination) is the same for all three.
• Intracellular protein localization IMP modified to protein localization to chromosome, telomeric region (GO:0070198).
• Protein binding (IPI) removed; telomeric/G4 DNA binding IBAs kept as non-core.

Falcon deep research (added after the initial review)

• The report agrees with the review: Rad50 is the ATP-dependent architectural MRN subunit, with nuclease activity supplied by Mre11 [file:DROME/rad50/rad50-deep-research-falcon.md "Rad50 contributes ATP-dependent structural control while Mre11 supplies nuclease activity"]. It also supports telomere capping via HOAP/HP1 recruitment [file:DROME/rad50/rad50-deep-research-falcon.md "HOAP and HP1 also failed to accumulate detectably at mutant polytene-chromosome"]. No annotation decisions changed.
• Review follow-up: the MRN telomere role is curated as GO:0016233 telomere capping across mre11, rad50 and nbs (IMP GO:0000723 rows MODIFY to capping; core_functions use capping), since the fly evidence is telomere fusion and loss of HOAP/HP1 at chromosome ends in a telomerase-free organism PMID:15296753.