Gene Ontology annotation through association of InterPro records with GO terms
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
UniProtKB entry for PP_2482
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UniProt identifies Q88K11 as a MoaA-like radical-SAM protein.
"DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR040064; MoaA-like."
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UniProt assigns a [4Fe-4S] cofactor by automated rule.
"CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};"
QuickGO GOA annotations for PP_2482
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The fetched table contains adjacent TreeGrafter rows for both exact MoaA and MoaC activities.
"UniProtKB Q88K11 PP_2482 enables GO:0061798 GTP 3',8'-cyclase activity molecular_function ECO:0007826 IEA GO_REF:0000118 PANTHER:PTN008586334 160488 Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) TreeGrafter Radical SAM core domain-containing protein 20260706
UniProtKB Q88K11 PP_2482 enables GO:0061799 cyclic pyranopterin monophosphate synthase activity molecular_function ECO:0007826 IEA GO_REF:0000118 PANTHER:PTN008586334 160488 Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) TreeGrafter Radical SAM core domain-containing protein 20260706"
OpenScientist gene research for PP_2482
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The report incorrectly promotes the uncertain Q88K11 paralog to canonical MoaA with an exact catalytic assignment.
"**PP_2482 (UniProt Q88K11) of *Pseudomonas putida* KT2440 encodes MoaA, a cytoplasmic radical S-adenosyl-L-methionine (radical SAM) enzyme that catalyzes the first committed step of molybdenum cofactor (Moco) biosynthesis.**"
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The report incorrectly calls PP_2482 an essential, nonredundant pathway gate despite the other KT2440 MoaA-family loci.
"MoaA (PP_2482) is a single-point, non-redundant gatekeeper: without it, none of these molybdoenzymes can be matured."
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The report presents unsaved residue-level analysis as decisive target evidence.
"Direct inspection of the 322-residue Q88K11 protein sequence confirms both metal-cluster signatures rather than relying on annotation alone."
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The report acknowledges that no direct PP_2482 experiment was found.
"1. **No direct experimental study of the *P. putida* ortholog.** All functional claims for PP_2482 specifically are made by inference."
Reproducible sequence comparison of KT2440 MoaA-family and MobA-like proteins
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Q88K11 lacks the two MoaA-specific InterPro records found on Q88E69.
"- The reviewed MoaA sequence Q88E69 is the only one of the three with the
MoaA-specific InterPro entries IPR013483 and IPR000385 in its UniProt record.
All three share broader MoaA-like/radical-SAM entries and PTHR22960."
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Q88K11 is 36.25% identical to canonical Q88E69 and is closer to Q88LG4.
"- Q88E69 is 36.25% identical to Q88K11 (PP_2482) and 37.85% identical to Q88LG4
(PP_1969) over aligned residue pairs. Q88K11 and Q88LG4 are 66.15% identical
to one another. This pattern supports a closer relationship between the two
unreviewed candidates than either has to canonical moaA, and does not support
assuming that all three are interchangeable copies."
UniProtKB entry for canonical KT2440 moaA
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Canonical KT2440 MoaA is specifically named as GTP 3',8'-cyclase.
"DE RecName: Full=GTP 3',8-cyclase {ECO:0000255|HAMAP-Rule:MF_01225};"
UniProtKB entry for KT2440 moaC