TrEMBL entry, 479 aa, bifunctional phosphomannose isomerase / GDP-mannose
pyrophosphorylase (type II PMI family; NCBIfam TIGR01479 GMP_PMI; PANTHER
PTHR46390:SF1). It sits at the 5' end of the A-band (common polysaccharide
antigen) O-antigen cluster next to gmd (PA5453) and rmd (PA5454). Paralogues
in PAO1: AlgA (PA3551, alginate; ~same domain architecture) and PslB
(PA2232, Psl; ORF488 in Rocchetta et al.).
[file:PSEAE/wbpW/wbpW-uniprot.txt "GMP_PMI"]
UniProt name and FUNCTION text for this TrEMBL entry come from ARBA: the name
"Alginate biosynthesis protein AlgA" and "Produces a precursor for alginate
polymerization" belong to the paralogue AlgA. wbpW serves A-band LPS (and,
redundantly, Psl), not alginate. Not cited as support anywhere in the review.
Rocchetta, Pacan and Lam 1998, PMID:9781879 (abstract only; no PMC).
- Complementation of E. coli PMI and GMP mutants (K30 capsule) by wbpW:
PMID:9781879
PMID:9781879
- wbpW chromosomal mutants make very little A-band:
PMID:9781879
- The abstract reports no enzyme assay with WbpW protein; the activity claim
rests on heterologous genetic complementation. The PseudoCAP IDA for
GDP-mannose biosynthetic process (the seed) is therefore most likely an
IGI-type experiment coded IDA. The term itself is right.
Byrd et al. 2009, PMID:19659934 (full text cached). wbpW and pslB are
redundant PMI/GMPs for A-band LPS and Psl:
PMID:19659934
PMID:19659934
PMID:19659934
The O antigen biosynthetic process row from this paper is coded IDA, but the
data are deletion mutants analysed by immunoblot: IMP (CONFIRMED
WRONG_EVIDENCE_CODE, full text read).
gmd (PA5453, same A-band pathway) has LPS and O antigen biosynthetic process
ACCEPTed; wbpW rows for the same processes are ACCEPTed in the same way.
GDP-mannose is the shared precursor for A-band (via Gmd, Rmd), Psl and (via
AlgA) alginate. algA is reviewed in the same batch and shares PAINT node
PTN000283179.
Seeds from .cache/paint/IBD.gaf; E. coli ManC (P24174) experimental rows from
QuickGO 2026-11-05: GO:0004475 IDA PMID:4908790 (EcoCyc); GO:0009298 EXP
PMID:12514000 (EcoCyc, acts_upstream_of_or_within).
| PTN node | node term | n_seeds | leaf IBAs | seed annotation (term, evidence, PMID) | verdict | rationale | other seeds | IBD stands without this seed? |
|---|---|---|---|---|---|---|---|---|
| PTN000283179 | GO:0009298 GDP-mannose biosynthetic process | 2 | 30 (26 taxa) | GO:0009298, IDA, PMID:9781879 | SOUND | WbpW complements E. coli PMI and GMP mutants and is needed for A-band, a GDP-D-Man-derived polymer; the term is right. Evidence is heterologous complementation (IGI), not IDA: PSEUDOCAP WRONG_EVIDENCE_CODE, PROBABLE. Term suits a ManC/AlgA-type bifunctional PMI-GMP clade. | E. coli ManC P24174 (EXP, PMID:12514000) | Yes |
The sister IBD at the same node, GO:0004475 mannose-1-phosphate
guanylyltransferase (GTP) activity, is seeded by ManC (IDA) and AlgA (P07874,
PseudoCAP IDA, purified enzyme); see genes/PSEAE/algA. Both node IBDs stand;
no GO_CENTRAL defect for PTN000283179 (none needed, so none recorded on
either gene).