Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Rapid coevolution of the nematode sex-determining genes fem-3 and tra-2.
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RNAi in C. elegans, C. briggsae and C. remanei shows the male-promoting function of fem-3 and its epistatic relationship with the upstream repressor tra-2 are conserved across all three species.
"the male-promoting function of fem-3 and its epistatic relationship with its female-promoting upstream repressor, tra-2, are conserved"
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FEM-3 binds TRA-2 directly, but the interaction is strictly species-specific and the FEM-3/TRA-2 interface is rapidly coevolving.
"the FEM-3 protein interacts with TRA-2 in each species, but in a strictly species-specific manner"
A CUL-2 ubiquitin ligase containing three FEM proteins degrades TRA-1 to regulate C. elegans sex determination.
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In C. elegans, TRA-1 is degraded by a CUL-2-based ubiquitin ligase in which FEM-1 is the substrate-recognition subunit and FEM-2 and FEM-3 are cofactors.
"TRA-1 is regulated by degradation mediated by a CUL-2-based ubiquitin ligase complex that contains FEM-1 as the substrate-recognition subunit, and FEM-2 and FEM-3 as cofactors"