Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Proteomic analysis of exosomes from human neural stem cells by flow field-flow fractionation and nanoflow liquid chromatography-tandem mass spectrometry.
A reference map of the human binary protein interactome.
ABHD8 antagonizes inflammation by facilitating chaperone-mediated autophagy-mediated degradation of NLRP3.
Affinage mechanistic annotation for ABHD8 (human)
What are ABHD8's IBA source proteins, and does any of them justify a lipid activity?
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Resolving all seven distinct WITH/FROM accessions on ABHD8's five lipid-activity IBAs, and querying each one's own GO evidence: SGD:S000004089 is ICT1_YEAST, whose UniProt recommended name is '1-acylglycerol-3-phosphate O-acyltransferase ICT1' - a named reviewed family member carrying the acyltransferase activity, not a paralog artefact. UniProtKB:Q8WTS1 is human ABHD5, which has 0 annotated active-site residues against ABHD8's 3 - so the 'ABHD5 is a fold without a function' analogy does not transfer to ABHD8; equally, ABHD5 itself has a demonstrated acyltransferase activity, so it is not the pseudoenzyme that analogy assumes.
"SGD:S000004089 resolves to ICT1_YEAST (Q12385, gene ICT1), whose UniProt recommended name is "1-acylglycerol-3-phosphate O-acyltransferase ICT1"."
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The five lipid-activity IBA rows have different WITH/FROM sets (GO:0006654 has 3 sources, GO:0042171 has 4, GO:0004620 and GO:0052689 have 5, GO:0055088 has 2), so a propagation_review copied between rows misstates what was inspected.
"| GO:0042171 | `AGI_LocusCode:AT4G24160`, `PANTHER:PTN008676419`, `SGD:S000004089`, `UniProtKB:Q8WTS1` |"
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Six of the seven distinct WITH/FROM sources across ABHD8's five lipid IBAs carry their own experimental evidence for at least one propagated term; only the PANTHER node does not. So these IBAs propagate from genuinely characterised enzymes in four organisms, which is why every one is MARK_AS_OVER_ANNOTATED rather than REMOVE. Two source identities were misdescribed before this was run: MGI:MGI:1915938 is mouse ABHD4, a paralog and NOT ABHD8's ortholog, and SGD:S000003342 is CLD1, a characterised cardiolipin-specific deacylase rather than a distant family member.
"| `SGD:S000003342` | P53264 (CLD1) — Cardiolipin-specific deacylase 1, mitochondrial | Swiss-Prot (reviewed) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | 0004620=IBA/IDA/IMP; 0006654=IBA; 0042171=IBA; 0052689=IBA/IDA/IMP; 0055088=IBA |"
Functional mechanisms underlying pleiotropic risk alleles at the 19p13.1 breast-ovarian cancer susceptibility locus.
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ABHD8 is a candidate target gene of the 19p13.1 breast/ovarian cancer susceptibility locus: risk SNPs physically contact the ABHD8 promoter and risk alleles increase its transactivation. This concerns regulation OF ABHD8 expression, not the protein's molecular function, so it supports no GO annotation here - but it explains why the gene is studied and is the main reason to expect its function to be pursued.
"Chromosome conformation capture identifies interactions between four candidate SNPs and ABHD8, and luciferase assays indicate six risk alleles increased transactivation of the ADHD8 promoter."
Comparative gene identification 58/α/β hydrolase domain 5 lacks lysophosphatidic acid acyltransferase activity.
Reassessing the Potential Activities of Plant CGI-58 Protein.