scat (scattered, Vps54, CG3766) notes

• GARP-defining subunit; male sterile; acroblast/acrosome defect [PMID:27481842 "The Vps54 (Scat) protein, a subunit of the GARP tethering complex, is a central factor in retrograde transport to the trans-Golgi."; "This acrosome formation defect was due to a fault in the trans-Golgi side of the acroblast ribbon."].
• Lerp (M6PR homolog) no longer reaches trans-Golgi in scat1 PMID:27481842.
• Scat-RFP at the trans side of the Golgi PMID:27481842.
• Individualization: scat1 forms hardly any investment cones PMID:27481842. Original isolation as an individualization mutant in PMID:9550716 (abstract only in cache).
• Binds Arl5-GTP with other GARP subunits PMID:25795912.
• VPS54 KD has a weaker effect on Tf recycling than syndetin KD PMID:25799061.

Decisions: GARP / retrograde transport ACCEPT (core); spermatogenesis IMP rows non-core; IDA endocytic recycling (PMID:25795912) MODIFY -> GO:0042147; Golgi-to-vacuole ISS from yeast over-annotated.

Deep research: falcon completed (genes/DROME/scat/scat-deep-research-falcon.md) after the review was first committed; checked for consistency.
- Deep research (falcon) adds: scat is required in motor neurons for TGN Syx16 organization and restrains NMJ growth (genetic interaction with Rab7); GARP prevents TGN sterol accumulation during dendrite regrowth (O'Brien et al. 2022, doi:10.1083/jcb.202112108) [file:DROME/scat/scat-deep-research-falcon.md "Loss or motor-neuron RNAi of scat made dStx16 staining more diffuse"]. These are not in GOA and were not added as NEW annotations.