Gene Ontology annotation through association of InterPro records with GO terms.
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt.
Automatic transfer of experimentally verified manual GO annotation data to orthologs by Ensembl Compara.
Automatic assignment of GO terms using logical inference, based on on inter-ontology links.
Gene Ontology annotation of human sequence-specific DNA binding transcription factors based on the TFClass classification.
ChAHP2 and ChAHP control diverse retrotransposons by complementary activities.
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ADNP2 immunoprecipitations recover CHD4 and HP1beta as the top two enrichments, defining a ChAHP2 complex; ADNP2 prefers HP1beta over HP1gamma, the reverse of ADNP's preference, and ADNP and ADNP2 do not co-purify with one another.
"The bait protein (ADNP2) was strongly enriched in the ADNP2 IPs compared with control, copurifying CHD4 and HP1β as the top two significantly enriched interactors"
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ChAHP2 reaches chromatin through HP1beta reading H3K9me3, not through DNA sequence recognition by ADNP2; mutating the PxVxL motif nearly abolishes binding to H3K9me3 target regions while leaving the mark itself intact.
"Mutations in the PxVxL motif resulted in a near-complete loss of ChAHP2 binding to H3K9me3-modified target regions, while H3K9me3 levels remained unchanged"
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No sequence motif could be defined that explains ADNP2's ChIP-seq distribution, and its peaks are overwhelmingly in repeats rather than at transcription start sites.
"In line with this sequence-agnostic recruitment mechanism, we were unable to define a sequence motif that would broadly explain the distribution and specificity of the ADNP2 ChIP-seq signal."
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ADNP2 loss de-represses ERV and LINE1 families without reducing H3K9me3, placing ChAHP2 downstream of heterochromatin rather than in its establishment.
"H3K9me3 levels at ADNP and ADNP2 peaks were not reduced by either individual or combined removal of these factors"
Silencing of the ADNP-family member, ADNP2, results in changes in cellular viability under oxidative stress.
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Mouse Adnp2 mRNA is enriched in embryonic brain and rises on P19 neuronal/glial differentiation - the expression pattern behind the GO:0007399 IEP that both human rows transfer.
"During development, a relatively
high level of ADNP2 gene expression was found in the embryonic mouse brain"
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siRNA knockdown of ADNP2 alters hydrogen peroxide toxicity in P19 embryonic carcinoma cells.
"ADNP2 deficiency significantly changed the toxicity
induced by hydrogen peroxide in P19 embryonic carcinoma cells"
Novel evolutionary-conserved role for the activity-dependent neuroprotective protein (ADNP) family that is important for erythropoiesis.
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BRG1/SMARCA4 was reported to interact with ADNP2, not only with ADNP.
"Brg1, an ADNP-interacting
chromatin-remodeling protein involved in erythropoiesis through regulation of
the globin locus, was shown here to interact also with ADNP2."
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Knockdown of ADNP2 blocks erythroid maturation in zebrafish embryos and in mouse erythroleukemia cells.
"Down-regulation/silencing of ADNP or ADNP2 in zebrafish embryos or
mouse erythroleukemia cells inhibited erythroid maturation"
Adnp2 promotes neural induction by antagonizing the ved and vent homeobox genes.
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Zebrafish adnp2a/adnp2b are required for neural induction and act as transcriptional repressors of ved/vent/vox.
"Adnp2 functions as a transcriptional repressor, and directly occupies
and suppresses the BMP-related ved/vent/vox homeobox genes"
NAP mechanisms of neuroprotection.
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The paper's subject is the synthetic eight-residue peptide NAPVSIPQ, a fragment of ADNP supplied exogenously, rather than the ADNP protein.
"An 8-amino-acid peptide, NAPVSIPQ (NAP), was identified as the smallest active
element of activity-dependent neuroprotective protein"
ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling.
Activity-dependent neuroprotective protein (ADNP) differentially interacts with chromatin to regulate genes essential for embryogenesis.
Activity-dependent neuroprotective protein recruits HP1 and CHD4 to control lineage-specifying genes.
Analysis of the human HP1 interactome reveals novel binding partners.
Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.
A High-Density Map for Navigating the Human Polycomb Complexome.
A reference map of the human binary protein interactome.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Genetic and Environmental Factors Co-Contributing to Behavioral Abnormalities in adnp/adnp2 Mutant Zebrafish.
Sex-Specific ADNP/NAP (Davunetide) Regulation of Cocaine-Induced Plasticity.
ADNP2 bioinformatics: NAP-peptide propagation audit, PxVxL motif with its compositional null, and IBD-seed evidence resolution