Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Probing the active site of Pseudomonas aeruginosa porphobilinogen synthase using newly developed inhibitors.
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Inhibitor kinetics and structures of the P. aeruginosa enzyme; Mg2+ seen in the active site.
"one of the determined protein structures provides for the first time structural evidence of a magnesium ion in the active site"
Cloning, mapping and functional characterization of the hemB gene of Pseudomonas aeruginosa, which encodes a magnesium-dependent 5-aminolevulinic acid dehydratase.
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hemB cloned by complementation of an E. coli hemB mutant.
"The hemB gene was cloned from Pseudomonas aeruginosa by functional complementation of an E. coli hemB mutant"
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Mg2+-dependent ALAD activity of the overexpressed enzyme measured directly.
"P. aeruginosa hemB was overexpressed in an E. coli hemB mutant using the phage T7 RNA polymerase system and its Mg(2+)-dependent activity was directly demonstrated"