Gene Ontology annotation based on UniPathway vocabulary mapping.

Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt.

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods.

PMID:9765566

The NADP-dependent methylene tetrahydromethanopterin dehydrogenase in Methylobacterium extorquens AM1.

"was identified to be the mtdA gene product"
"The purified enzyme catalyzed the dehydrogenation of methylene H4MPT with NADP+ rather than with NAD+"
"It also catalyzed the"
"dehydrogenation of methylene tetrahydrofolate (methylene H4F) with NADP+"
"catalytic efficiency (Vmax/Km) were approximately 20-fold lower than with"
"dehydrogenation of methylene H4F (E0 = -300 mV) was fully reversible"

PMID:8144463

Genetics of the serine cycle in Methylobacterium extorquens AM1: identification of sgaA and mtdA and sequences of sgaA, hprA, and mtdA.

"This open reading frame was identified as the gene required for the synthesis of 5,10-methylenetetrahydrofolate dehydrogenase."
"this enzyme plays an integral role in methylotrophic metabolism in M. extorquens"
"either in formaldehyde oxidation or as part of the serine cycle"

file:METEA/mtdA/mtdA-deep-research-falcon.md

Falcon (Edison Scientific) deep research report for mtdA (P55818), Methylorubrum extorquens AM1

"NADP+-dependent methylene-pterin dehydrogenase"
"catalytic efficiency for methylene-H4F is reported to be"
"MtdA shows only minor sequence identity to"
"major formaldehyde oxidation/detoxification route"
"MtdA is one of two methylene dehydrogenases in this network (MtdA and MtdB)"
"oxidizing methylene-H4MPT to methenyl-H4MPT"
"as part of a route ultimately leading to formate/CO2"
"a mechanistic link between formaldehyde processing and the H4F C1 pool used for assimilation"
"generating methenyl-/formyl-H4F for biosynthetic C1 metabolism"
"membrane fraction lacked NAD(P)-dependent methylene-H4MPT dehydrogenase activity"
"consistent with MtdA not being membrane-associated"
"null mutants in mtdA could not be obtained"
"supporting that mtdA is likely essential under tested conditions"
"did not restore growth on methanol"