Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Molecular characterization of LhpR in control of hydroxyproline catabolism and transport in Pseudomonas aeruginosa PAO1.
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The gene at PA1255 is called lhpK in this paper and named a "Hyp/Pro racemase" from sequence; the genetic evidence is that D-Hyp induction of the lhpA promoter is abolished only in the lhpA lhpK double mutant.
"Induction of the lhpA promoter by d-Hyp was completely abolished in the lhpA lhpK double mutant devoid of two epimerases, while the induction effect of l-Hyp remained unchanged"
Functional characterization of aconitase X as a cis-3-hydroxy-L-proline dehydratase.
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Assigns PA1255 (PaLhpL) a bifunctional trans-3-hydroxy-L-proline dehydratase and 2-epimerase activity; the paper's own main subject is the aconitase-X enzyme PaLhpI, which acts on the cis isomer.
"We herein demonstrated that PaLhpL gene (PA1255; Fig. 1d) in the L-hydroxyproline gene cluster encodes a bifunctional dehydratase and 2-epimerase toward trans-3-hydroxy-L-proline as the leading candidate"
Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens.
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Assayed P. aeruginosa proline-racemase-family proteins directly; the genuine hydroxyproline-2-epimerase (PaHyPRE) epimerizes OH-L/D-Pro, whereas members with substituted catalytic couples showed neither proline racemase nor hydroxyproline epimerase activity. Swiss-Prot cites this paper for the absence of t4LHyp epimerase and proline racemase activity in Q9I489.
"B. pseudomallei and P. aeruginosa recombinant proteins presented no measurable PRAC activity but demonstrated strong epimerization of OH-L/D-Pro behaving as genuine OH-Pro epimerases."
Prediction and characterization of enzymatic activities guided by sequence similarity and genome neighborhood networks.
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Genome-neighbourhood study of the proline racemase superfamily that defines the trans-3-hydroxy-L-proline dehydratase (t3HypD) activity and includes Q9I489 among its sequences; source of the UniProt statement that lhpL is induced by trans-4- and trans-3-hydroxy-L-proline.
"The reactions catalyzed by proline racemase (ProR), 4R-hydroxyproline 2-epimerase (4HypE), and trans-3-hydroxy-L-proline dehydratase (t3HypD) and the metabolic pathways in which they participate."
UniProt entry Q9I489 (T3HPD_PSEAE)
lhpL bioinformatics - PAINT node PTN002433936 composition and propagation