GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:20305657

Self-poisoning of Mycobacterium tuberculosis by targeting GlgE in an alpha-glucan pathway.

Experimental work in Mycobacterium established the conserved four-step TreS-Mak-GlgE-GlgB route and identifies maltose 1-phosphate as the GlgE donor; it supports the pathway architecture but is not a direct assay of Pseudomonas TreSB.

"We describe a new pathway from trehalose to alpha-glucan in Mycobacterium tuberculosis comprising four enzymatic steps mediated by TreS, Pep2, GlgE (which has been identified as a maltosyltransferase that uses maltose 1-phosphate) and GlgB."

file:PSEPK/treSB/treSB-deep-research-openscientist.md

OpenScientist functional-annotation report for treSB

The report corroborates the fused TreS and maltokinase architecture, exact two-reaction assignment, and position upstream of GlgE while distinguishing direct target evidence from ortholog-derived mechanism.

file:PSEPK/treSB/treSB-uniprot.txt

UniProtKB entry for treSB (Q88FN0)

The unreviewed record assigns two reactions to one 1106-aa protein: EC 5.4.99.16/RHEA:15145 for maltose-trehalose interconversion and EC 2.7.1.175/RHEA:31915 for ATP-dependent maltose phosphorylation.

file:interpro/panther/PTHR10357/PTHR10357-paint.tsv

PANTHER PAINT annotations for PTHR10357

A bacterial PTHR10357 ancestral node carries GO:0047471 with IBD evidence, seeded by the characterized Mycobacterium TreS P9WQ19.

file:interpro/panther/panther-members.tsv

Canonical PANTHER member index

Q88FN0 and the PAINT seed P9WQ19 are both verified members of PTHR10357:SF219, whose official label is MALTOSE ALPHA-D-GLUCOSYLTRANSFERASE.

"Q88FN0 PTHR10357:SF219"

file:PSEPK/glgE/glgE-uniprot.txt

UniProtKB entry for adjacent glgE (Q88FM9)

GlgE consumes the alpha-maltose 1-phosphate produced by TreSB.

"Maltosyltransferase that uses maltose 1-phosphate (M1P)"