Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
The human mitochondrial ISCA1, ISCA2, and IBA57 proteins are required for [4Fe-4S] protein maturation.
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Established that ISCA1, ISCA2, and IBA57 are required for mitochondrial [4Fe-4S] protein maturation
"The human mitochondrial ISCA1, ISCA2, and IBA57 proteins are required for [4Fe-4S] protein maturation"
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RNAi depletion causes loss of [4Fe-4S] enzyme activities (aconitase, lipoic acid synthase)
"The activities of mitochondrial [4Fe-4S] proteins, including aconitase, respiratory complex I, and lipoic acid synthase, were diminished following depletion of the three proteins"
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Confirmed mitochondrial localization of ISCA2
"We conclude that all three ISC proteins are located in mitochondria"
Formation of [4Fe-4S] clusters in the mitochondrial iron-sulfur cluster assembly machinery.
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ISCA2 binds either [2Fe-2S] or [4Fe-4S] cluster in a dimeric state
"ISCA2 binds either [2Fe-2S] or [4Fe-4S] cluster in a dimeric state"
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ISCA1:ISCA2 heterodimeric complex receives [2Fe-2S] clusters from GRX5
"two molecules of [2Fe-2S](2+) GRX5 donate their cluster to a heterodimeric ISCA1/ISCA2 complex"
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The complex assembles [4Fe-4S] clusters from two [2Fe-2S] precursors
"the two GRX5-donated [2Fe-2S](2+) clusters generate a [4Fe-4S](2+) cluster"
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Established the ISCA1:ISCA2 complex as the functional unit for [4Fe-4S] assembly
"These findings imply that such heterodimeric complex is the functional unit in mitochondria"
A proteome-scale map of the human interactome network.
Structural properties of [2Fe-2S] ISCA2-IBA57: a complex of the mitochondrial iron-sulfur cluster assembly machinery.
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ISCA2 forms homodimer that provides core interface for IBA57 interaction
"ISCA2-IBA57 complex with ISCA2 providing the homodimerization core interface"
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Characterized [2Fe-2S]-bridged ISCA2-IBA57 heterocomplex structure by SAXS
"The [2Fe-2S] cluster is out of the ISCA2 core while being shared with IBA57 in the dimer"
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Complex forms dimer of dimers (heterotetramer)
"dimeric [2Fe-2S] ISCA2-IBA57 hetero-complex is a physiologically relevant species"
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Defined molecular basis for pathogenic IBA57 Arg146Trp mutation
"The specific interaction pattern identified from the dimeric [2Fe-2S]2+ ISCA2-IBA57 structural model allowed us to define the molecular grounds of the pathogenic Arg146Trp mutation of IBA57"
A reference map of the human binary protein interactome.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Defects in the Maturation of Mitochondrial Iron-Sulfur Proteins: Biophysical Investigation of the MMDS3 Causing Gly104Cys Variant of IBA57.
Multimodal cell maps as a foundation for structural and functional genomics.
Formation of 4Fe-4S cluster on ISCA1:ISCA2
Transfer of Fe-S clusters to SDHB