Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
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DET1 family functions (cullin-RING ubiquitin ligase complex membership, ubiquitin protein ligase binding, ubiquitin-like ligase-substrate adaptor activity, protein ubiquitination, positive regulation of proteasomal degradation) are conserved across the DET1 phylogenetic group (PANTHER PTHR13374 / PTN000332575).
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
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SwissProt keyword-derived (SPKW) annotations present in the goa_uniprot snapshot used by the SPKW-PLANTS project but removed from the current GOA release after GOA retired the keyword2GO pipeline for cellular organisms.
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For DET1, the keyword "Phytochrome signaling pathway" mapped to two red/far-red light terms (GO:0009585, GO:0010017); these are mis-placed/over-specific for a downstream nuclear repressor and are better replaced by negative regulation of photomorphogenesis (GO:0010100).
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Phenotype of the tomato high pigment-2 mutant is caused by a mutation in the tomato homolog of DEETIOLATED1.
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The tomato HP-2 gene encodes the tomato homolog of the nuclear Arabidopsis protein DEETIOLATED1 (DET1); HP genes were proposed to be negative regulators of phytochrome signal transduction.
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det1 mutations cause constitutive de-etiolation in darkness; the tomato hp-2 phenotype is strictly dependent on active phytochrome, placing DET1 downstream within phytochrome signaling.
The tomato dark green mutation is a novel allele of the tomato homolog of the DEETIOLATED1 gene.
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The dark-green (dg) and hp-2(j) mutations are allelic at the tomato DET1 locus; DET1 is involved in the signal transduction cascade of light perception and morphogenesis.
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dg mutants show an exaggerated photomorphogenic de-etiolation response, and the DET1 locus is strongly linked to the photomorphogenic (hypocotyl-length) response.
CUL4 associates with DDB1 and DET1 and its downregulation affects diverse aspects of development in Arabidopsis thaliana.
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Arabidopsis CUL4 assembles with DDB1, RBX1, DET1 and DDB2 in vitro and in planta to form a functional cullin4-RING E3 ligase; cullins are scaffolding subunits that facilitate ubiquitination of target proteins.
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The IntAct interaction underlying the IPI "protein binding" annotation for tomato DET1 is with DDB1 (UniProtKB:Q8LGH4), a component of the CUL4-based ligase.
UniProt entry DET1_SOLLC (Q9ZNU6) - Light-mediated development protein DET1.
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UniProt annotates DET1 as a component of light signal transduction machinery and a negative regulator of fruit pigmentation, probably acting in the CDD complex (with DDB1); repression of photomorphogenesis is probably mediated by ubiquitination and degradation of photomorphogenesis-promoting factors such as HY5.
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Subcellular location is Nucleus; the protein carries two predicted nuclear localization signals and belongs to the DET1 family (IPR019138/PF09737), with the six-hairpin glycosidase superfamily fold (IPR008928) being structural only.
Deep-research report (falcon / Edison Scientific Literature) - functional annotation of tomato DET1 (Q9ZNU6).
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Synthesizes the tomato DET1/hp2 literature, concluding DET1 is a nuclear regulatory scaffold/adaptor of a CUL4-DDB1-DET1 (CRL4) E3 ligase that ubiquitinates and degrades transcription factors (notably SlGLK2), repressing photomorphogenesis and controlling plastid/pigment accumulation in fruit.
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Documents DET1 participation in the CDD complex (DDB1, COP10), nuclear (and cytoplasmic) localization in tomato YFP assays, and the high-pigment biofortification phenotype (elevated carotenoids, tocopherols, flavonoids) of DET1-downregulated fruit.