GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000118

TreeGrafter-generated GO annotations

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

file:PSEPK/fadA__Q88L01/fadA__Q88L01-uniprot.txt

UniProtKB reviewed entry for Pseudomonas putida KT2440 fadA

UniProt identifies FadA as the cytoplasmic beta subunit of the FadBA heterotetramer and assigns the terminal beta-oxidation thiolase reaction.
The PANTHER assignment is to the FadA-specific beta-oxidation subfamily rather than to the thiolase family root.

"PANTHER; PTHR43853:SF11; 3-KETOACYL-COA THIOLASE FADA; 1."

file:PSEPK/fadA__Q88L01/fadA__Q88L01-notes.md

Curation notes for Pseudomonas putida KT2440 fadA

Appended audit qualifies the older categorical phenylacetate exclusion and documents the PP_3275/PP_3280 source discrepancy.

file:PSEPK/paaJ/paaJ-uniprot.txt

UniProtKB entry Q88HS3 (Pseudomonas putida KT2440 paaJ)

file:ECOLI/fadA/fadA-uniprot.txt

UniProtKB entry P21151 (FADA_ECOLI), experimentally characterized E. coli orthologue

PMID:368024

Five different enzymatic activities are associated with the multienzyme complex of fatty acid oxidation from Escherichia coli.

PMID:334745

Evidence for a complex of three beta-oxidation enzymes in Escherichia coli: induction and localization.

file:PSEPK/fadB/fadB-source-analysis/primary-scope.md

KT2440 beta-oxidation primary assay and source-scope analysis

Direct KT2440 fitness is distinguished from enzyme kinetics, inferred complex compatibility and the uncertain PP_1893 native contribution.