Shared GAPDH family evidence (TDH1/TDH2/TDH3 are paralogous NAD+-dependent phosphorylating GAPDHs, EC 1.2.1.12):
- Activity partition among paralogs PMID:3905788; all three active PMID:3905788.
- Same relative expression on glucose and ethanol (supports gluconeogenic as well as glycolytic role) PMID:3905788.
- Tdh2/Tdh3 homotetramers; Tdh3 lower Vmax PMID:3905788.
- Cell wall + cytosol PMID:11158358; PMID:11158358.
- Side activity: NAD(P)H hydratase [UniProt:P00360 "FUNCTION: As a side activity, catalyzes the hydration of the"]; NADP binding IEA from IPR006424 judged over-annotation for an NAD-specific GAPDH.
- Peripheral mitochondrial pool PMID:16962558; minor lipid-particle co-fractionation PMID:10515935.
- Melatonin affinity pull-down hit PMID:31708896; weak affinity-capture evidence marked over-annotated.
- NO/S-nitrosation and apoptosis (TDH2/TDH3 IMP) PMID:17726063 (abstract only; kept non-core).
TDH1-specific:
- Stationary-phase/stress-induced minor isoform [UniProt:P00360 "INDUCTION: Expressed when cells enter stationary phase, due to glucose"].
- IPI mitochondrion rows are with ENO1 (SGD:S000003486) and ENO2 (SGD:S000001217): co-IP with anti-enolase PMID:16962558.
Decisions: ACCEPT GAPDH activity, glycolysis, gluconeogenesis, cytosol/cytoplasm; MODIFY generic oxidoreductase -> GO:0004365 and obsolete GO:0061620 -> GO:0006096; REMOVE bifid shunt; MARK_AS_OVER_ANNOTATED NADP binding, melatonin binding; KEEP_AS_NON_CORE mitochondrion, lipid droplet, cell wall, PM, NAD binding, glucose metabolic process.