The Differential Translation Capabilities of the Human DHFR2 Gene Indicates a Developmental and Tissue-Specific Endogenous Protein of Low Abundance.
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Purified HEK293 mitochondria have DHFR reductase activity, and targeted proteomics detects canonical DHFR but not DHFR2 peptides, assigning mitochondrial reductase activity to DHFR and revising the earlier attribution to DHFR2/DHFRL1.
"This data provides compelling evidence that DHFR (and not DHFR2) is the reductase activity in human mitochondria."
Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Atomic structures of human dihydrofolate reductase complexed with NADPH and two lipophilic antifolates at 1.09 a and 1.05 a resolution.
Structure determination of tetrahydroquinazoline antifolates in complex with human and Pneumocystis carinii dihydrofolate reductase: correlations between enzyme selectivity and stereochemistry.
Novel boron-containing, nonclassical antifolates: synthesis and preliminary biological and structural evaluation.
Correlations of inhibitor kinetics for Pneumocystis jirovecii and human dihydrofolate reductase with structural data for human active site mutant enzyme complexes.
Multiple conformers in active site of human dihydrofolate reductase F31R/Q35E double mutant suggest structural basis for methotrexate resistance.
The former annotated human pseudogene dihydrofolate reductase-like 1 (DHFRL1) is expressed and functional.
Identification of a de novo thymidylate biosynthesis pathway in mammalian mitochondria.
An active second dihydrofolate reductase enzyme is not a feature of rat and mouse, but they do have activity in their mitochondria.
Serine hydroxymethyltransferase anchors de novo thymidylate synthesis pathway to nuclear lamina for DNA synthesis.
Unusual transient- and steady-state kinetic behavior is predicted by the kinetic scheme operational for recombinant human dihydrofolate reductase.
Human endothelial dihydrofolate reductase low activity limits vascular tetrahydrobiopterin recycling.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Multimodal cell maps as a foundation for structural and functional genomics.
The nucleotide sequence of the cDNA coding for the human dihydrofolic acid reductase.
Specific binding of human dihydrofolate reductase protein to dihydrofolate reductase messenger RNA in vitro.
Structure-based design and synthesis of lipophilic 2,4-diamino-6-substituted quinazolines and their evaluation as inhibitors of dihydrofolate reductases and potential antitumor agents.
DHFR gene expression is stimulated by E2F1
DHFR dimer reduces FOLA to DHF
DHFR dimer binds DHFR inhibitors
Identification and characterization of an inborn error of metabolism caused by dihydrofolate reductase deficiency.
Dihydrofolate reductase deficiency due to a homozygous DHFR mutation causes megaloblastic anemia and cerebral folate deficiency leading to severe neurologic disease.
Falcon deep research report for human DHFR