Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Chromatin localization of nucleophosmin organizes ribosome biogenesis.
Saccharomyces cerevisiae Nip7p is required for efficient 60S ribosome subunit biogenesis.
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Budding-yeast Nip7 (same PANTHER subfamily PTHR23415:SF4 as Q1MTQ9) associates with free 60S particles but not with mature ribosomes, and is therefore a non-ribosomal assembly factor.
"A fraction of Nip7p cosedimented through sucrose gradients with free 60S ribosomal subunits but not with 80S monosomes or polysomal ribosomes, indicating that it is not a ribosomal protein."
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Nip7 depletion delays 27S pre-rRNA processing and causes a 60S subunit deficit.
"Delayed processing of 27S pre-rRNA appeared to be the cause of reduced synthesis of 25S rRNA relative to 18S rRNA, which may be responsible for the deficit of 60S subunits in these cells."
Structural insights into the interaction of the Nip7 PUA domain with polyuridine RNA.
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The Nip7 PUA domain binds RNA, with a polyuridine preference conserved between archaeal and eukaryotic (budding-yeast) Nip7.
"This work provides experimental verification that the PUA domain mediates Nip7 interaction with RNA and reveals that the preference for interaction with polyuridine sequences is conserved in Archaea and eukaryotic Nip7 proteins."
The NIP7 protein is required for accurate pre-rRNA processing in human cells.
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Human NIP7 is nuclear and associates with pre-ribosomal particles.
"The NIP7 protein is restricted to the nuclear compartment and co-sediments with complexes with molecular masses in the range of 40S-80S, suggesting an association to nucleolar pre-ribosomal particles."
UniProtKB Q1MTQ9: curated sequence, features and source attribution
RCSB PDB 8ESQ polymer-entity metadata: Q1MTQ9 is entity 43, author chain l