Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Combined Automated Annotation using Multiple IEA Methods
Molecular basis of glutathione reductase deficiency in human blood cells.
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GR deficiency in patient blood cells abolishes glutathione reductase activity; a 2246-bp deletion removes the dimerization domain and a G330A substitution impairs thermostability, establishing the molecular basis of GR deficiency and supporting the enzyme's catalytic function.
"Red blood cells and leukocytes of the patients in family 1 did not"
Large-scale proteomics and phosphoproteomics of urinary exosomes.
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Large-scale LC-MS/MS proteomics of human urinary exosomes identified GSR among 1132 proteins, supporting an incidental exosome-proteome localization.
"the analysis identified 1132 proteins unambiguously"
In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
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Shotgun proteomics of prostatic-secretion (urinary) exosomes detected GSR among ~900 proteins, an incidental secreted-vesicle localization.
"In pooled EPS-urine exosome samples, ~900 proteins were detected."
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Familial deficiency of glutathione reductase in human blood cells.
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Classic description of familial glutathione reductase deficiency in human erythrocytes and leukocytes, with hemolytic crises after eating fava beans; establishes the enzyme's activity and clinical relevance.
"A virtually complete absence of glutathione reductase activity was found in the"
GSR catalyzes glutathione (oxidized) + NADPH + H+ => 2 glutathione (reduced) + NADP+
glutathione (oxidized) + NADPH + H+ => 2 glutathione (reduced) + NADP+
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Cytosolic glutathione reductase catalyzes reduction of GSSG by NADPH to form two GSH; deficiency can cause hemolytic anemia.
"Cytosolic glutathione reductase catalyzes the reaction of glutathione (oxidized)"
NFE2L2-dependent GSR gene expression
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GSR contributes to cellular redox balance by reducing GSSG to GSH; its expression is regulated in part by the KEAP1:NFE2L2 (NRF2) antioxidant-response pathway.
"Glutathione reductase (GSR) contributes to the redox balance in a cell by"