Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniPathway vocabulary mapping
Combined Automated Annotation using Multiple IEA Methods
Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440
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The P. putida KT2440 genome encodes genes for aromatic compound degradation pathways including the beta-ketoadipate pathway via the cat regulon.
"Pseudomonas putida is a metabolically versatile saprophytic soil bacterium... Sequence analysis of the 6.18 Mb genome of strain KT2440 reveals diverse transport and metabolic systems."
A second chromosomal copy of the catA gene endows Pseudomonas putida mt-2 with an enzymatic safety valve for excess of catechol
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CatA is the dominant catechol 1,2-dioxygenase in P. putida, with CatA2 serving as a metabolic safety valve for excess catechol at high concentrations. Induction of the ortho pathway by benzoate in KT2440 leads to catechol surplus counteracted by CatA2.
"The data consistently indicated that induction of the ortho pathway by benzoate plasmid-less strain P. putida KT2440 led to catechol surplus, the toxicity of which at high concentrations being counteracted by CatA2."
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P. putida mt-2 has a second chromosomal copy of the catA gene (catA2) located downstream of the ben operon encoding an additional catechol 1,2-dioxygenase.
"P. putida mt-2 has a second chromosomal copy of the catA gene (named catA2) located downstream of the ben operon that encodes an additional catechol-1,2-dioxygenase."
Genomic analysis of the aromatic catabolic pathways from Pseudomonas putida KT2440
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KT2440 has catechol (cat genes) and protocatechuate (pca genes) branches of the beta-ketoadipate pathway, plus homogentisate and phenylacetate pathways for aromatic compound catabolism.
"predicted the existence of at least four main pathways for the catabolism of central aromatic intermediates, that is, the protocatechuate (pca genes) and catechol (cat genes) branches of the beta-ketoadipate pathway, the homogentisate pathway (hmg/fah/mai genes) and the phenylacetate pathway (pha genes)."
Generation of a catR deficient mutant of P. putida KT2440 that produces cis, cis-muconate from benzoate at high rate and yield
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CatR is the transcriptional regulator of the cat operon. A point mutation in the DNA binding domain of catR abolishes cat operon induction. The ben operon contains PP_3166 (catA2), a second catechol 1,2-dioxygenase.
"Transcriptome analysis showed that the cat operon was not induced in P. putida KT2440-JD1 in the presence of 5mM benzoate, due to a point mutation in the highly conserved DNA binding domain of the transcriptional regulator (catR) of the cat operon. The ben operon was highly expressed in the presence of benzoate in the mutant and its parental strain. This operon contains PP_3166 (catA2), which was shown to be a second catechol 1,2-dioxygenase besides catA."