Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Mapping the Arabidopsis organelle proteome.
Two Arabidopsis thaliana Golgi alpha-mannosidase I enzymes are responsible for plant N-glycan maturation.
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Recombinant MNS1 (AtMANIb) and MNS2 are 1-deoxymannojirimycin-sensitive alpha-mannosidase I enzymes converting Man8/Man9GlcNAc2 to Man5GlcNAc2, by a hydrolytic route distinct from that of mammalian MANI.
"AtMANIs employ hydrolytic pathways distinct from those of mammalian MANIs"
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mns1 mns2 double mutants accumulate Man8GlcNAc2 and lack complex/hybrid N-glycans.
"the manIa manIb double mutant lines produced Man(8)GlcNAc(2) as the predominant N-glycan and lacked plant-specific complex and hybrid N-glycans"
Class I alpha-mannosidases are required for N-glycan processing and root development in Arabidopsis thaliana.
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MNS1/MNS2 remove three alpha-1,2-mannoses from Man8GlcNAc2 and are Golgi-localized; the mns1 mns2 mns3 triple mutant has short swollen roots and altered cell walls.
"MNS1/2 readily removed three alpha1,2-mannose residues from Man(8)GlcNAc(2)"
Isolation and proteomic characterization of the Arabidopsis Golgi defines functional and novel components involved in plant cell wall biosynthesis.
Putative glycosyltransferases and other plant Golgi apparatus proteins are revealed by LOPIT proteomics.
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Golgi and TGN were merged into a single classifier class, so TGN/endosome calls from this study do not resolve those compartments from Golgi.
"The Golgi apparatus and the TGN proteins were combined into one class because they have a similar fractionation profile."
Separating Golgi Proteins from Cis to Trans Reveals Underlying Properties of Cisternal Localization.
Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum-Associated Degradation of Misfolded Glycoproteins.
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mns1 mns2 does not suppress bri1-5 or bri1-9; MNS1-MNS3 are dispensable for glycan-dependent ERAD, which requires ER-resident MNS4/MNS5.
"These data indicate that MNS4 and MNS5 are required for the turnover of the membrane-anchored ERAD substrates BRI1-5 and BRI1-9, whereas MNS1 to MNS3 are dispensable for this process."
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MNS1/MNS2 preferentially trim the A-branch terminal alpha-1,2-mannose.
"MNS1 and MNS2 preferentially cleave off a terminal α1,2-linked Man residue from the A-branch"
Trimming of N-Glycans by the Golgi-Localized α-1,2-Mannosidases, MNS1 and MNS2, Is Crucial for Maintaining RSW2 Protein Abundance during Salt Stress in Arabidopsis.
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mns1 mns2 roots are salt-hypersensitive with compromised cellulose synthesis and reduced RSW2/KOR abundance; a downstream consequence of defective glycan maturation.
"abolishment of two functionally redundant mannosidases, MNS1 and MNS2, responsible for α-1,2-mannose trimming on the A and C branches of plant N-glycans"
A context-independent N-glycan signal targets the misfolded extracellular domain of Arabidopsis STRUBBELIG to endoplasmic-reticulum-associated degradation.
UniProtKB record for Arabidopsis MNS1
PAINT IBD assertions for PANTHER family PTHR11742