Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
Analysis of a vinculin homolog in a sponge (phylum Porifera) reveals that vertebrate-like cell adhesions emerged early in animal evolution.
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TLN has the conserved talin domain organization, plus an additional predicted PTB domain.
"As in their mammalian counterparts, Op talin has an N-terminal FERM domain followed by a talin middle domain, vinculin-binding sites, and an I/WLEQ domain."
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The tested TLN fragment is a synthetic peptide of residues 598-621, homologous to the mouse talin vinculin-binding site.
"In a MUSCLE alignment Mm talin 605–628 correspond to residues 598–621 in Op talin"
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The TLN peptide binds VIN1 and activates VIN1 binding to F-actin.
"In the presence of Op talin peptide, full-length Op vinculin bound to F-actin filaments"