Oxygen levels rapidly modulate Pseudomonas aeruginosa social behaviours via substrate limitation of PqsH.
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Purified MBP-PqsH converts HHQ to PQS using NADH and O2 in vitro (terminal step of PQS biosynthesis).
"we show that PqsH catalyses the terminal step in PQS production, synthesizing PQS in vitro using the substrates 2-heptyl-4-quinolone (HHQ), NADH and oxygen"
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Steady-state kinetics confirm HHQ, NADH and oxygen as substrates; NADPH is a poorer electron donor.
"Steady state kinetic analysis confirmed HHQ, NADH, and oxygen as substrates of MBP-PqsH, consistent with its predicted role as an HHQ hydroxylating monooxygenase"
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PqsH belongs to the flavin-dependent NAD(P)H/O2 aromatic hydroxylase family; FAD content itself was not measured.
"PqsH is a member of a family of flavin-dependent monooxygenases that utilize NAD(P)H and oxygen to catalyze hydroxylation of aromatic substrates"
Functions required for extracellular quinolone signaling by Pseudomonas aeruginosa.
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pqsH is required for PQS synthesis and its transcription depends on LasR.
"Since pqsH is required for PQS synthesis, this finding accounts for a previous observation that PQS production requires LasR function"
Signal Synthase-Type versus Catabolic Monooxygenases: Retracing 3-Hydroxylation of 2-Alkylquinolones and Their N-Oxides by Pseudomonas aeruginosa and Other Pulmonary Pathogens.
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PqsH and the catabolic ortholog AqdB catalyse the same HHQ 3-hydroxylation.
"Hydroxylation of the PQS precursor 2-heptylquinolin-4(1H)-one (HHQ) by the signal synthase PqsH boosts AQ quorum sensing. Remarkably, the same reaction, catalyzed by the ortholog AqdB"
Composition of PANTHER PTHR45934 tree under PAINT node PTN008173107
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PTN008173107 is the PTHR45934 family root (297 leaves); 294 leaves are in a Viridiplantae clade under a horizontal-transfer node shared with PqsH and Streptomyces SCO1338.
"So 294 of the 297 leaves are green-plant proteins"
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods