Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Multidrug resistance-like genes of Arabidopsis required for auxin transport and auxin-mediated development.
Two homologous ATP-binding cassette transporter proteins, AtMDR1 and AtPGP1, regulate Arabidopsis photomorphogenesis and root development by mediating polar auxin transport.
-
Native atmdr1 and atpgp1 mutants alter light-dependent hypocotyl growth and pigment accumulation; this source interprets the phenotypes in the context of polar auxin transport.
"loss-of-function atmdr1 and atpgp1 mutants display hypersensitivity to far-red, red, and blue-light inhibition of hypocotyl elongation, reduced chlorophyll and anthocyanin accumulation"
PIN proteins perform a rate-limiting function in cellular auxin efflux.
Mapping the Arabidopsis organelle proteome.
-
LOPIT organelle proteomics assigned ABCB19 to the plasma membrane.
"enabling confident and simultaneous localization of 527 proteins to the endoplasmic reticulum, Golgi apparatus, vacuolar membrane, plasma membrane, or mitochondria and plastids"
Interactions among PIN-FORMED and P-glycoprotein auxin transporters in Arabidopsis.
-
Interaction of ABCB19/PGP19 with PIN1 in yeast two-hybrid and co-immunoprecipitation assays; PGP19 stabilizes PIN1 in sterol/sphingolipid-enriched membrane domains.
"Specific PGP-PIN interactions were seen in yeast two-hybrid and coimmunoprecipitation assays."
Separating the roles of acropetal and basipetal auxin transport on gravitropism with mutations in two Arabidopsis multidrug resistance-like ABC transporter genes.
-
mdr1 (abcb19) mutations reduce acropetal (rootward, central-cylinder) auxin transport in roots by 80%.
"Mutations in Multidrug Resistance-Like1 (MDR1) reduced acropetal auxin transport in roots by 80% without affecting basipetal transport."
Mutations in Arabidopsis multidrug resistance-like ABC transporters separate the roles of acropetal and basipetal auxin transport in lateral root development.
-
Reduced acropetal transport in mdr1 lateral roots slows their growth. The phenotype reflects altered auxin (or brassinosteroid) distribution caused by loss of ABCB19 transport activity.
"impaired acropetal auxin transport due to mutation of the MDR1 gene caused 21% of nascent lateral roots to arrest their growth"
Auxin regulates Arabidopsis anther dehiscence, pollen maturation, and filament elongation.
-
Auxin transport (ABCB1/ABCB19) contributes to preanthesis filament elongation. The phenotype reflects altered auxin (or brassinosteroid) distribution caused by loss of ABCB19 transport activity.
"auxin transport contributes to the independent regulation of preanthesis filament elongation"
Putative glycosyltransferases and other plant Golgi apparatus proteins are revealed by LOPIT proteomics.
The ATP-binding cassette transporter ABCB19 regulates postembryonic organ separation in Arabidopsis.
Global Analysis of Membrane-associated Protein Oligomerization Using Protein Correlation Profiling.
-
The membrane-profiling source does not establish a cytosolic ABCB19 pool.
"Over 150 proteins had a complicated localization pattern, and were clearly partitioned between cytosolic and membrane-associated pools."
Connective auxin transport contributes to strigolactone-mediated shoot branching control independent of the transcription factor BRC1.
-
Loss of ABCB19 partially suppresses the branching of strigolactone mutants. The phenotype reflects altered auxin (or brassinosteroid) distribution caused by loss of ABCB19 transport activity.
"Because loss of ABCB19 in strigolactone mutant backgrounds reduces branching in intact plants, we wondered whether abcb19 mutants were strigolactone resistant."
An ATP-Binding Cassette Transporter, ABCB19, Regulates Leaf Position and Morphology during Phototropin1-Mediated Blue Light Responses.
-
ARBA rule for response to blue light. The phenotype reflects altered auxin (or brassinosteroid) distribution caused by loss of ABCB19 transport activity.
"abcb19 mutants develop irregularly wavy rosette leaves that are less sensitive to blue light-mediated leaf flattening"
Arabidopsis TWISTED DWARF1 regulates stamen elongation by differential activation of ABCB1,19-mediated auxin transport.
-
The double transporter mutant has altered pollen viability/number; this is not an isolated ABCB19 mitotic assay.
"Most of the described developmental defects in twd1 are shared with the abcb1 abcb19 mutant"
Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export.
-
ATP binding by the two nucleotide-binding domains.
"Bioactive brassinosteroids are potent activators of ABCB19 ATP hydrolysis activity, and transport assays showed that ABCB19 transports brassinosteroids."
Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1.
-
ABCB19-mediated BR export contributes to cellular brassinosteroid responses.
"ABCB19 functions as a BR exporter and, together with its close homolog ABCB1, positively regulates BR signaling"
phot1 inhibition of ABCB19 primes lateral auxin fluxes in the shoot apex required for phototropism.
ABCB19/PGP19 stabilises PIN1 in membrane microdomains in Arabidopsis.
https://biblio.ugent.be/publication/01HTMBK3P3PPNB73YFCMFXP3ZZ/file/01HTMC3Z7XJ6XPXCM124Z4RD35.pdf
-
ABCB19 has cellular IAA export activity despite negative purified-protein assays.
"One possible explanation 135
is that ABCB19-mediated auxin efflux requires some endogenous factors that are missing in 136
the in vitro system."
Target-specific inspection of PMID:28887381
-
ABCB19 is classified as membrane-associated, without a dual-localized cytosolic call in the inspected S1 rows.
"Both rows have `True membrane = True`, blank `Dual Localized`, and `SUBAcon localization = plasma membrane`."
Arabidopsis TWISTED DWARF1 regulates stamen elongation by differential activation of ABCB1,19-mediated auxin transport.
-
The abcb1 abcb19 double mutant has a native pollen phenotype.
"both pollen viability and pollen number were significantly reduced in the tw1 and the abcb1abcb19 double mutant but not in investigated pin mutants"
-
ABCB19 is present in rapidly elongating stamen filament tissues.
"ABCB19–GFP expression was very strong in stamen filaments at flower stage 12 and was localized in both epidermal and provascular cells"
Two Homologous ATP-Binding Cassette Transporter Proteins, AtMDR1 and AtPGP1, Regulate Arabidopsis Photomorphogenesis and Root Development by Mediating Polar Auxin Transport
-
Native AtMDR1/ABCB19 mutant anthocyanin accumulation was assayed under far-red and blue illumination, not a UV stimulus.
"Seedlings (approximately 100) were grown in continuous far-red or blue light for 4 d before anthocyanin extraction."