Use of the ND evidence code for Gene Ontology (GO) terms
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
RGD ISO annotations to rat from other mammalian species
Synaptic and endosomal localization of active gamma-secretase in rat brain.
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Aph-1aL co-fractionates with gamma-secretase components and activity in rat brain endosome-enriched fractions, synaptic membranes and purified synaptic vesicles.
"We conclude that both the synaptic membrane and the synaptic vesicle fractions have high γ-secretase activity and that the γ-secretase components are found also in highly pure synaptic vesicles."
Aph-1 associates directly with full-length and C-terminal fragments of gamma-secretase substrates.
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Endogenous rat Aph-1 co-immunoprecipitates with endogenous Jagged-1 in E13 rat embryo microsomes; Aph-1 binds APP holoprotein and CTFs, requiring His-171/His-197.
"we were able to observe an interaction between endogenous Jagged-1 and endogenous Aph-1"
Differential contribution of the three Aph1 genes to gamma-secretase activity in vivo.
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Mouse Aph1a knockout is embryonic lethal with Notch-like defects; Aph1b/c are dispensable for viability.
"Aph1A-/- embryos show a lethal phenotype characterized by angiogenesis defects in the yolk sac, neuronal tube malformations, and mild somitogenesis defects."
S-palmitoylation of gamma-secretase subunits nicastrin and APH-1.
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APH-1 is S-palmitoylated, affecting stability and raft association but not substrate processing.
"APH-1 is S-palmitoylated at Cys(182) and Cys(245)"
UniProt entry A0ABK0KYA7 (rat Aph1a)