Gene Ontology annotation based on Enzyme Commission mapping
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding.
Towards a proteome-scale map of the human protein-protein interaction network.
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Rual et al. CCSB-HI1 proteome-scale yeast two-hybrid map records a LRSAM1 protein-protein interaction. High-throughput dataset; the interaction supports only bare protein binding, not a specific molecular function.
"Systematic mapping of protein-protein interactions, or 'interactome' mapping, was initiated in model organisms"
A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration.
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Lim et al. yeast two-hybrid network for 54 ataxia/Purkinje-cell-degeneration proteins reports an interaction involving LRSAM1. Provides only bare protein-binding evidence, not a specific molecular function.
"we developed an interaction network for 54 proteins involved in 23 inherited ataxias"
Regulation of Tsg101 expression by the steadiness box: a role of Tsg101-associated ligase.
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Tal (LRSAM1) polyubiquitinates lysine residues in the C-terminus of uncomplexed TSG101, targeting it for proteasomal degradation; accessibility to these lysines is masked when TSG101 is engaged with other ESCRT-I subunits. Establishes Tal as a regulator of ESCRT-I (TSG101) steady-state levels.
"Tal polyubiquitinates lysine residues in the C-terminus of uncomplexed Tsg101, resulting in proteasomal degradation. However, accessibility to these lysines is prevented by the presence of the other ESCRT-I proteins."
Analysis of the human E2 ubiquitin conjugating enzyme protein interaction network.
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Markson et al. yeast two-hybrid map of human E2/E3-RING interactions records LRSAM1 (a RING E3) among the E3 partners tested against 35 E2 conjugating enzymes. Supports LRSAM1 acting through canonical E2-E3 RING chemistry but provides only bare protein-binding-level evidence.
"yeast two-hybrid (Y2H) screens were combined with true homology modeling methods to generate a high-density map of human E2/E3-RING interactions"
A comprehensive framework of E2-RING E3 interactions of the human ubiquitin-proteasome system.
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van Wijk et al. Y2H framework of 35 E2s vs 250 RING-type E3s (including LRSAM1) reports LRSAM1 E2-RING interactions. Consistent with LRSAM1 functioning as a canonical RING E3 partnering with E2 conjugating enzymes; bare protein-binding-level evidence only.
"We performed a global yeast-two hybrid screen to study the specificity of interactions between catalytic domains of the 35 human E2s with 250 RING-type E3s."
Genome-wide YFP fluorescence complementation screen identifies new regulators for telomere signaling in human cells.
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Lee et al. genome-wide bimolecular fluorescence complementation screen for telomere-interactome regulators reports an LRSAM1 interaction with a core telomeric protein. High-throughput screen; provides only bare protein-binding evidence, no specific molecular function for LRSAM1.
"we developed an arrayed screening strategy based on protein complementation to systematically investigate protein-protein interactions in live human cells, and performed a large-scale screen for regulators of telomeres"
The LRR and RING domain protein LRSAM1 is an E3 ligase crucial for ubiquitin-dependent autophagy of intracellular Salmonella Typhimurium.
The MEKK1 PHD ubiquitinates TAB1 to activate MAPKs in response to cytokines.
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Charlaftis et al. characterize MEKK1's PHD domain as an atypical E3 that ubiquitinates TAB1; an LRSAM1 interaction is reported in the associated interactome data. Not directly informative about LRSAM1 function; supports only bare protein binding.
"The MEKK1 PHD binds and mediates the transfer of Lys63-linked poly-Ub, using the conjugating enzyme UBE2N, onto TAB1 to regulate TAK1 and MAPK activation"
A proteome-scale map of the human interactome network.
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Rolland et al. HI-II-14 proteome-scale yeast two-hybrid map reports LRSAM1 protein-protein interactions. High-throughput dataset; provides only bare protein-binding evidence, no specific molecular function.
"A proteome-scale map of the human interactome network."
PHF23 (plant homeodomain finger protein 23) negatively regulates cell autophagy by promoting ubiquitination and degradation of E3 ligase LRSAM1.
A novel missense mutation of CMT2P alters transcription machinery.
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Hu et al. identify a novel Cys694Arg missense mutation altering a conserved cysteine in the LRSAM1 RING domain in a family with Charcot-Marie-Tooth type 2P (CMT2P). The mutation causes axonal degeneration in a CRISPR/Cas9 neuronal cell model and perturbs RNA-binding protein pathways.
"The affected members in our family cosegregated with a novel missense mutation Cys694Arg that alters a highly conserved cysteine in the RING domain. This mutation leads to axonal degeneration in the in vitro neuronal cell line."
A reference map of the human binary protein interactome.
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Luck et al. HuRI reference binary interactome (yeast two-hybrid) records LRSAM1 interactions. High-throughput dataset; provides only bare protein-binding evidence, no specific molecular function.
"A reference map of the human binary protein interactome."
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
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Haenig et al. neurodegenerative disease interactome maps LRSAM1 interactions relevant to the CMT2P (Charcot-Marie-Tooth type 2P) phenotype. High-throughput dataset; provides only bare protein-binding evidence.
"Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains."
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
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Huttlin et al. BioPlex 3.0 dual (HEK293T/HCT116) affinity-purification-mass-spectrometry interactome records LRSAM1 interactions. High-throughput dataset; provides only bare protein-binding evidence, no specific molecular function.
"BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins."