Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Differentiation-associated expression of ceramidase isoforms in cultured keratinocytes and epidermis.
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All five ceramidase isoforms are expressed in keratinocytes/epidermis, with acidic and alkaline ceramidase activities increasing with differentiation; alkaline ceramidase-1 (ACER1) is essentially epidermis-restricted.
"alkaline CDase-1 occurs almost exclusively in epidermis"
Upregulation of the human alkaline ceramidase 1 and acid ceramidase mediates calcium-induced differentiation of epidermal keratinocytes.
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haCER1 (ACER1) hydrolyses very-long-chain ceramides to generate sphingosine in a Ca2+-dependent manner; its Ca2+-induced upregulation mediates growth arrest and differentiation of human epidermal keratinocytes.
"haCER1 catalyzed the hydrolysis of very long-chain ceramides to generate sphingosine (SPH). This in vitro activity required Ca2+."
Role of alkaline ceramidases in the generation of sphingosine and its phosphate in erythrocytes.
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Alkaline ceramidase activity generates sphingosine (the sphingosine-1-phosphate precursor) from ceramide; sphingosine is produced only from ceramide hydrolysis by ceramidases.
"SPH is only generated from the hydrolysis of ceramides via ceramidases."
Alkaline ceramidase 2 (ACER2) and its product dihydrosphingosine mediate the cytotoxicity of N-(4-hydroxyphenyl)retinamide in tumor cells.
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Alkaline ceramidase catalyses hydrolysis of (dihydro)ceramides to generate the sphingoid base; ACER2 hydrolyses dihydroceramides to dihydrosphingosine.
"ACER2, which catalyzes the hydrolysis of dihydroceramides to generate DHS"
A reference map of the human binary protein interactome.
ACER1 hydrolyzes ceramide (endoplasmic reticulum)
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ACER1 catalyses hydrolysis of ceramide to a free fatty acid and sphingosine at the ER membrane; it acts on C20:0-C24:0 ceramides and is mainly expressed in skin.
"ACER1 (alkaline ceramidase 1) catalyzes the reversible hydrolysis of ceramide to yield a free fatty acid and sphingosine"
UniProtKB Q8TDN7 (ACER1_HUMAN)
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Endoplasmic reticulum alkaline ceramidase hydrolysing ceramides to sphingosine and free fatty acids at alkaline pH; multi-pass ER membrane protein; skin-specific; catalytic zinc; Ca2+-dependent; EC 3.5.1.23.
"Endoplasmic reticulum ceramidase that catalyzes the hydrolysis of ceramides into sphingosine and free fatty acids at alkaline pH"