Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
The genetic basis of constitutive and herbivore-induced ESP-independent nitrile formation in Arabidopsis.
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At3g16400 (AtNSP1) is required for constitutive and P. rapae-induced simple-nitrile formation in Col-0 rosette leaves.
"a line with a T-DNA insertion in the second exon of At3g16400 (SALK_072600) produced no or only trace amounts of simple nitriles of the endogenous glucosinolates in rosette leaf homogenates"
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Purified NSP1 forms simple nitriles (not epithionitriles or thiocyanates) from several glucosinolates with myrosinase, and its activity is not dependent on added iron.
"Thus, simple nitrile formation by AtNSP1 is not dependent on the presence of Fe 2+ or Fe 3+ ion."
Nitrile-specifier proteins involved in glucosinolate hydrolysis in Arabidopsis thaliana.
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Recombinant At3g16400 (called AtNSP3 in this paper) has nitrile-specifier activity on allyl- and benzylglucosinolate.
"Assays with crude bacterial extracts containing AtNSP1 ( At5g48180 ) or AtNSP3 ( At3g16400 ) recombinant proteins also resulted in the production of 2-phenylacetonitrile"
A proteomic strategy for global analysis of plant protein complexes.
In Planta Determination of the mRNA-Binding Proteome of Arabidopsis Etiolated Seedlings.
Global Analysis of Membrane-associated Protein Oligomerization Using Protein Correlation Profiling.
Glucosinolate Content in Dormant and Germinating Arabidopsis thaliana Seeds Is Affected by Non-Functional Alleles of Classical Myrosinase and Nitrile-Specifier Protein Genes.
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nsp1 mutants have altered glucosinolate content in seeds and during germination, which suggests a role in glucosinolate turnover.
"The time course of total glucosinolate content during ten days of germination depended on functional NSP1."
NSP-Dependent Simple Nitrile Formation Dominates upon Breakdown of Major Aliphatic Glucosinolates in Roots, Seeds, and Seedlings of Arabidopsis thaliana Columbia-0.
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NSP1 provides most nitrile-forming activity in seedlings and, with NSP3, in roots of Col-0.
"simple nitrile formation upon tissue disruption depended almost entirely on NSP2 in seeds and mainly on NSP1 in seedlings."
Crystal structure of the nitrile-specifier protein NSP1 from Arabidopsis thaliana.
Structural diversification during glucosinolate breakdown: mechanisms of thiocyanate, epithionitrile and simple nitrile formation.
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Specifier proteins are proposed to be Fe(2+)-dependent lyases. NSPs form simple nitriles irrespective of side chain, and AtNSP1 is a monomer.
"NSPs promote simple nitrile formation irrespective of the glucosinolate side chain"
Iron is a centrally bound cofactor of specifier proteins involved in glucosinolate breakdown.
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Iron is bound by specifier proteins through an EXXXDXXXH triad. This was shown for the paralog At3g16390 (called AtNSP3 here), not directly for NSP1. The paper notes AtNSP1's N-terminal JAL domain.
"In addition, AtNSP1 possesses an N-terminal JAL domain"
The antagonist function of Arabidopsis WRKY53 and ESR/ESP in leaf senescence is modulated by the jasmonic and salicylic acid equilibrium.