Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Characterization of recombinant yeast exo-beta-1,3-glucanase (Exg 1p) expressed in Escherichia coli cells.
-
Recombinant EXG1 expressed in E. coli has exo-beta-1,3-glucanase activity and beta-glucosidase activity toward small beta-1,3, beta-1,6, and other beta-glucosidic substrates, while showing poor activity on polymeric laminaran and pustulan.
"Kinetic analyses indicate that the enzyme prefers small substrates such as laminaribiose, gentiobiose, and pNPG rather than polysaccharide substrates, such as laminaran or pustulan."
Phospholipid transfer protein Sec14 is required for trafficking from endosomes and regulates distinct trans-Golgi export pathways.
-
Exg1 remains secreted from sec14(ts) cells and is detected by mass spectrometry among proteins secreted into the culture medium.
"Identity was determined by mass spectrometry and included Hsp150, Cts1, Scw4, Scw10, Exg1, Cis3, and Ygp1, proteins known to be secreted by S. cerevisiae"
One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy.
-
The SWAT-GFP acceptor screen constitutively expressed N-terminally tagged endomembrane proteins from the SpNOP1 promoter, an ectopic setup for the signal-peptide-bearing secreted Exg1 glycoprotein.
"To demonstrate the SWAT approach we constructed an acceptor library using an acceptor module that included the constitutive SpNOP1 promoter 19 and a GFP tag (SWAT-GFP)."
Structural Analysis of Laminarin-Derived Oligosaccharides Produced by Transglycosylation of Exo-β-1,3-glucanase ScEXG1 from Saccharomyces cerevisiae.
-
Recombinant His-tagged ScEXG1 hydrolyzes beta-1,3 glucan and can transglycosylate laminaribiose into beta-1,6-containing tri- and tetrasaccharides under low-enzyme in vitro conditions.
"Exo-β-1,3-glucanase EXG1 (ScEXG1) from Saccharomyces cerevisiae hydrolyzes β-1,3-glucans and exhibits transglycosylation activity"
Regulation of cell wall beta-glucan assembly: PTC1 negatively affects PBS2 action in a pathway that includes modulation of EXG1 transcription.
-
EXG1 overexpression lowers beta-1,6-glucan, while EXG1 deletion modestly increases beta-1,6-glucan and killer-toxin sensitivity.
"Overexpression of EXG1 on a 2 mu plasmid led to reduction in a cell wall beta 1,6-glucan and caused killer resistance in wild type cells; while the exg1 delta mutant displayed modest increases in killer sensitivity and beta 1,6-glucan levels."
New potential cell wall glucanases of Saccharomyces cerevisiae and their involvement in mating.
-
Exg1 was identified by N-terminal sequencing among soluble cell-wall proteins released from intact cells.
"three of these proteins were identified as the known proteins beta-exoglucanase 1 (Exg1p), beta-endoglucanase (Bgl2p), and chitinase (Cts1p)"