Use of the ND evidence code for Gene Ontology (GO) terms
Annotation inferences using phylogenetic trees
Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress.
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Genome-wide GFP screen scoring subcellular localization changes during DNA replication stress; one localization-change class captures proteins moving away from the bud neck / bud tip, and HU/MMS caused a decrease in bud-neck/bud-tip localization. DSF2 is one of the proteins scored (in the supplementary datasets), which is the basis for its high-throughput (HDA) bud-tip localization annotation.
"one that reflects movement away from the budneck or bud tip"
Falcon deep-research report on S. cerevisiae DSF2 (YBR007C)
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Domain-based synthesis: characterized Sel1-like-repeat proteins act as non-enzymatic adaptor/scaffold proteins; on this basis DSF2 is best interpreted as a scaffold/adaptor at the bud neck. The report also raises (as unverified inferences) a possible link to the Cbk1/RAM network and specific phosphosites; these are not adopted as supported claims here.
"non-enzymatic adaptor or scaffold protein"
Suppressor analysis of the mpt5/htr1/uth4/puf5 deletion in Saccharomyces cerevisiae.
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Screen for deletion suppressors that rescue the temperature-sensitivity of an mpt5 (puf5) deletion identified dsf1 (YEL070W) and dsf2 (YBR007C), among others. This is the origin of the DSF2 gene name and its only dedicated functional study; it establishes a genetic suppressor relationship, not a molecular function.
"dsf1 (YEL070W), dsf2 (YBR007C)"
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The mpt5 deletion strain is hydroxyurea (HU) sensitive; DSF2 loss partially suppresses this HU sensitivity (per UniProt disruption phenotype).
"The Deltampt5 disruptant was also hydroxyurea (HU) sensitive"