GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

PMID:21444755

WRB is the receptor for TRC40/Asna1-mediated insertion of tail-anchored proteins into the ER membrane.

Identifies WRB/CHD5 as the ER membrane receptor for TRC40/Asna1 and maps the WRB coiled-coil domain as the TRC40 docking site; WRB is ER-resident, not nuclear.

PMID:23041287

Molecular machinery for insertion of tail-anchored membrane proteins into the endoplasmic reticulum membrane in mammalian cells.

WRB and CAML synergistically insert TA proteins into the ER membrane and together constitute the mammalian TRC40 receptor complex.

PMID:27226539

Tail-anchored Protein Insertion in Mammals: FUNCTION AND RECIPROCAL INTERACTIONS OF THE TWO SUBUNITS OF THE TRC40 RECEPTOR.

In-vitro-synthesized CAML and WRB together are sufficient to confer TA-insertion competence to liposomes; the two subunits are reciprocally dependent.

PMID:31417168

The WRB Subunit of the Get3 Receptor is Required for the Correct Integration of its Partner CAML into the ER.

WRB acts catalytically to assist CAML topogenesis; without sufficient WRB, CAML adopts aberrant topoforms that cluster and are degraded by the proteasome.

PMID:32187542

Differential Modes of Orphan Subunit Recognition for the WRB/CAML Complex.

The WRB/CAML complex is an essential insertase for tail-anchored proteins; WRB can correct the topology of CAML in vitro and in cells.

PMID:32296183

A reference map of the human binary protein interactome.

High-throughput Y2H (HuRI) interactome; source of the IntAct protein binding partners of GET1.

PMID:32910895

Structural Basis of Tail-Anchored Membrane Protein Biogenesis by the GET Insertase Complex.

Cryo-EM of the human WRB/CAML/TRC40 complex; WRB and CAML form a phosphatidylinositol-stabilized heterotetramer that binds the GET3 homodimer, with an insertion mechanism related to YidC and the EMC.

PMID:9544840

Identification and characterization of a new human cDNA from chromosome 21q22.3 encoding a basic nuclear protein.

Original cDNA characterization reporting predominant nuclear immunofluorescence localization; not reproduced and superseded by later ER membrane localization.

PMID:34264263

Capture and delivery of tail-anchored proteins to the endoplasmic reticulum.

Comprehensive review of the GET/TRC pathway; GET1/WRB with CAMLG/GET2 forms the ER membrane receptor-insertase that receives TA substrates from the cytosolic ATPase GET3/TRC40 and mediates their insertion into the ER bilayer.

PMID:36640319

The Get1/2 insertase forms a channel to mediate the insertion of tail-anchored proteins into the ER.

Reconstitution shows the Get1/2 (WRB/CAML) complex forms a dynamic aqueous channel (~2.5 nm, corresponding to two Get1/2 complexes) that is sealed by Get3 and whose channel activity is required to release TA proteins from Get3 for insertion; functions as both insertase and translocase for C-terminal hydrophilic tails.

PMID:37963916

The GET insertase exhibits conformational plasticity and induces membrane thinning.

Structures, simulations and functional data of human and C. thermophilum Get1/Get2/Get3 show the GET insertase fold is conserved, induces local lipid bilayer thinning near the hydrophilic groove to lower the insertion barrier, and that gating between Get2 helix alpha3' and Get3 drives conformational changes across the Get1/Get2 heterotetramer.

file:human/GET1/GET1-uniprot.txt

UniProt entry O00258 (GET1_HUMAN), Guided entry of tail-anchored proteins factor 1

GET1/WRB is the ER membrane receptor for GET3/TRC40 in the GET complex (GET1/WRB + CAMLG/GET2 + GET3/TRC40); multi-pass ER membrane protein required for post-translational delivery of TA proteins to the ER.