Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Electronic Gene Ontology annotations created by ARBA machine learning models
Overexpression of human diacylglycerol acyltransferase 1, acyl-coa:cholesterol acyltransferase 1, or acyl-CoA:cholesterol acyltransferase 2 stimulates secretion of apolipoprotein B-containing lipoproteins in McA-RH7777 cells.
Mutation in apolipoprotein B associated with hypobetalipoproteinemia despite decreased binding to the low density lipoprotein receptor.
-
Human mutant LDL has reduced LDLR affinity; the full source-specific cholesterol-transfer assay remains unresolved.
"R3480P LDL
bound with lower affinity to the LDL receptor than non-carrier LDL in vitro"
Hepatitis C virus nonstructural proteins inhibit apolipoprotein B100 secretion.
The effect of heparin on structural and functional properties of low density lipoproteins.
Transcriptomic and proteomic analyses of rhabdomyosarcoma cells reveal differential cellular gene expression in response to enterovirus 71 infection.
Induction of dendritic cell-like phenotype in macrophages during foam cell formation.
C-reactive protein-bound enzymatically modified low-density lipoprotein does not transform macrophages into foam cells.
Chlamydia pneumoniae induces macrophage-derived foam cell formation via PPAR alpha and PPAR gamma-dependent pathways.
Mechanisms of inhibition by apolipoprotein C of apolipoprotein E-dependent cellular metabolism of human triglyceride-rich lipoproteins through the low density lipoprotein receptor pathway.
-
Human IDL contains both apoB100 and apoE, whose receptor contributions can be distinguished.
"IDL is a lipoprotein that
contains both apoB-100 and apoE."
A physical interaction network of dengue virus and human proteins.
Proteomics analysis of cardiac extracellular matrix remodeling in a porcine model of ischemia/reperfusion injury.
Proprotein convertase subtilisin/kexin type 9 interacts with apolipoprotein B and prevents its intracellular degradation, irrespective of the low-density lipoprotein receptor.
Apolipoprotein B is a new target of the GDNF/RET and ET-3/EDNRB signalling pathways.
In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
LDL receptor/lipoprotein recognition: endosomal weakening of ApoB and ApoE binding to the convex face of the LR5 repeat.
-
Defined apoB site peptides bind LDLR repeat LR5 in NMR/SPR assays; intact-particle uptake is outside this assay scope.
"We have used NMR and SPR to characterize the complexes formed by LR5 and
three peptides encompassing the putative binding regions of ApoB (site A and
site B) and ApoE."
Novel missense MTTP gene mutations causing abetalipoproteinemia.
Association between a specific apolipoprotein B mutation and familial defective apolipoprotein B-100.
Novel Abetalipoproteinemia Missense Mutation Highlights the Importance of the N-Terminal β-Barrel in Microsomal Triglyceride Transfer Protein Function.
-
ApoB association is separable from MTP PDI binding and lipid-transfer activity.
"Although D169V was expressed in the endoplasmic
reticulum and interacted with apolipoprotein B (apoB) 17, it was unable to bind
protein disulfide isomerase, transfer lipids, and support apoB secretion."
Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
TANGO1 and Mia2/cTAGE5 (TALI) cooperate to export bulky pre-chylomicrons/VLDLs from the endoplasmic reticulum.
-
ApoB-containing particles are recruited as cargo to ER exit sites by TANGO1/TALI.
"TANGO1 binds TALI, and both interact
with apolipoprotein B (ApoB) and are necessary for the recruitment of
ApoB-containing lipid particles to ER exit sites for their subsequent export."
TREM2 Binds to Apolipoproteins, Including APOE and CLU/APOJ, and Thereby Facilitates Uptake of Amyloid-Beta by Microglia.
Structure-function analyses of microsomal triglyceride transfer protein missense mutations in abetalipoproteinemia and hypobetalipoproteinemia subjects.
AUP1 (Ancient Ubiquitous Protein 1) Is a Key Determinant of Hepatic Very-Low-Density Lipoprotein Assembly and Secretion.
Assay of total plasma apolipoprotein B concentration in human subjects.
-
Human apoB is detected in chylomicrons, VLDL and LDL fractions.
"The assay measures not only the ApoB content of
beta-lipoproteins (low density lipoproteins [LDL]) but also that contained in
the other lipoproteins in plasma."
Plasma lipoproteins: apolipoprotein structure and function.
Sulfhydryl chemistry and solubility properties of human plasma apolipoprotein B.
Identification of disulfide-linked apolipoprotein species in human lipoproteins.
-
Disulfide-linked apoB100–apoAII occurs in human VLDL and LDL.
"the demonstration of the existence of the disulfide-linked species
apolipoprotein B-100:A-II and B-100:C-IIToronto in the very low density and low
density lipoproteins"
Interaction between ApoB and hepatic lipase mediates the uptake of ApoB-containing lipoproteins.
-
Hepatic lipase binds apoB in the reported fragment and LDL assays.
"On a ligand blot, HL bound to apoB26, 48, and 100 but not to apoE or
apoAI."
LDLR:LDL complex [coated vesicle membrane] => LDLR:LDL complex [endosome membrane]
LDLR:LDL complex => LDLR + LDL
LDL + LDLR => LDL:LDLR complex
LDL:LDLR complex [plasma membrane] => LDL:LDLR complex [clathrin-coated vesicle] (LDLRAP1-independent)
nascent chylomicron [endoplasmic reticulum lumen] => nascent chylomicron [extracellular]
chylomicron remnant:apoE complex + LDLR => chylomicron remnant:apoE:LDLR complex
nascent chylomicron + spherical HDL:apoC-II:apoC-III:apoE =>spherical HDL + chylomicron
TG-depleted chylomicron + spherical HDL => chylomicron remnant + spherical HDL:apoA-I:apoA-II:apoA-IV:apoC-II:apoC-III
chylomicron remnant:apoE:LDLR complex [plasma membrane] => chylomicron remnant:apoE:LDLR complex [clathrin-coated vesicle] (LDLRAP1-dependent)
Degradation of newly synthesized ApoB-48
chylomicron remnant + apoE => chylomicron remnant:apoE complex
ApoB-48:TG:PL complex + 100 triacylglycerols + ApoA-I + ApoA-IV => nascent chylomicron
chylomicron => TG-depleted chylomicron + 50 long-chain fatty acids + 50 diacylglycerols
ApoB-48 + 40 triacylglycerol + 60 phospholipid => ApoB-48:TG:PL complex
apolipoprotein(a) + LDL => Lp(a)
OLR1 binds to oxidized LDL
COLEC12 (SCARA4) binds ligands
Cytosolic CMs translocate to extracellular region
SCARB1 (SR-BI, CLA-1) binds ligands
STAB1 (FEEL-1) binds ligands
STAB2 (FEEL-2) binds ligands
STAB2:ligand is endocytosed
Platelet glycoprotein IV:ligand (CD36:ligand) is endocytosed
STAB1:ligand is endocytosed
SCARF1:ligand is endocytosed
atREs binds to nascent CM
LPL hydrolyses TGs from mature CMs
Nascent CMs transform into mature CMs
LRPs transport extracellular CR:atREs:HSPG:apoE to cytosol
NREH hydrolyses atREs to atROL and FAs
CR:atREs binds apoE and HSPG
NREH hydrolyses atREs (HSPG:apoE) to atROL and FAs
MSR1:ligand (SCARA1:ligand, SR-A:ligand) is endocytosed
SCARB1:ligand is endocytosed
CETP-mediated lipid exchange: LDL gains cholesterol ester
COLEC12:ligand is endocytosed
FGR binds and phosphorylates LRP8
LDLR is bound by DAB2 and ARH
LIPA hydrolyses sterol esters to sterols and fatty acids
pre-VLDL binds lipids to form VLDL
pre-VLDL translocates from RER membrane to SER lumen
VLDL binds APOC1 and APOC4
VLDL translocates from SER lumen to extracellular region
MTTP lipidates APOB-100, forming a pre-VLDL
F- and N- BAR domain proteins bind the clathrin-coated pit
CLASP proteins and cargo are recruited to the nascent clathrin-coated pit
Clathrin recruits PIK3C2A
Clathrin-associated PIK3C2A phosphorylates PI(4)P to PI(3,4)P2
SNX9 recruits components of the actin polymerizing machinery
BAR domain proteins recruit dynamin
SYNJ hydrolyze PI(4,5)P2 to PI(4)P
Endophilins recruit synaptojanins to the clathrin-coated pit
HSPA8-mediated ATP hydrolysis promotes vesicle uncoating
Clathrin recruits auxilins to the clathrin-coated vesicle
Auxilin recruits HSPA8:ATP to the clathrin-coated vesicle
Dynamin-mediated GTP hydrolysis promotes vesicle scission
Dissociation of clathrin-associated proteins
oxLDL:CD36 binds TLR4:TLR6
Dissociation of AAK1 and dephosphorylation of AP-2 mu2
RAB5 and GAPVD1 bind AP-2
LDL translocates from endosome lumen to lysosome lumen
FAM20C phosphorylates FAM20C substrates
The structure of apolipoprotein B100 from human low-density lipoprotein.
Structure of apolipoprotein B100 bound to the low-density lipoprotein receptor.
-
The reported receptor-complex structure identifies apoB100–LDLR binding interfaces; exact primary Methods remain unread.
"One binding interface is formed between several
small-ligand-binding modules of LDLR and a series of basic patches that are
scattered along a β-belt formed by apoB100, encircling LDL."