Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Basolateral sorting signals differ in their ability to redirect apical proteins to the basolateral cell surface.
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ASGR1 (H1) is a basolateral plasma membrane protein whose cytoplasmic domain contains a basolateral sorting signal.
"We have generated chimeric proteins with the cytoplasmic domain of either the asialoglycoprotein receptor H1 or the transferrin receptor, two basolateral proteins, fused to the transmembrane and exoplasmic segments of aminopeptidase N, an apical protein, and analyzed them in Madin-Darby canine kidney cells."
Cloning, mapping, and characterization of a human homologue of the yeast longevity assurance gene LAG1.
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Yeast two-hybrid screening and GST pull-down assays identified interactions between LASS2 (CERS2) and membrane-associated receptors or transporters.
"Yeast two-hybrid screening and glutathione S-transferase pull-down assays showed that the LASS2 protein interacts with several membrane-associated receptors or transporters."
Nonpalmitoylated human asialoglycoprotein receptors recycle constitutively but are defective in coated pit-mediated endocytosis, dissociation, and delivery of ligand to lysosomes.
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The human hepatic asialoglycoprotein receptor (composed of subunits H1/ASGR1 and H2/ASGR2) internalizes desialylated glycoproteins at the plasma membrane via the clathrin-coated pit pathway for lysosomal degradation.
"The hepatic asialoglycoprotein receptor (ASGP-R) internalizes desialylated glycoproteins via the clathrin-coated pit pathway and mediates their delivery to lysosomes for degradation. The human ASGP-R contains two subunits, H1 and H2."
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Palmitoylation of ASGR1 (Cys-36) and ASGR2 (Cys-54 and Cys-58) is required for efficient clathrin-dependent endocytosis, ligand dissociation, and lysosomal delivery.
"We conclude that palmitoylation of the ASGP-R is required for its efficient endocytosis of ligand by the clathrin-dependent endocytic pathway and, in particular, for the proper dissociation and delivery of ligand to lysosomes."
Toward an understanding of the protein interaction network of the human liver.
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High-throughput yeast two-hybrid screen of human liver proteins mapping binary protein-protein interactions.
"In this study, we map the interactions of an unbiased selection of 5026 human liver expression proteins by yeast two-hybrid technology and establish a human liver protein interaction network (HLPN) composed of 3484 interactions among 2582 proteins."
Asialoglycoprotein receptor 1 mediates productive uptake of N-acetylgalactosamine-conjugated and unconjugated phosphorothioate antisense oligonucleotides into liver hepatocytes.
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ASGR1 is a cell-surface C-type lectin receptor that binds terminal GalNAc and mediates receptor-mediated endocytosis of desialylated glycoproteins and GalNAc-conjugated antisense oligonucleotides into hepatocytes.
"GalNAc conjugation confers high affinity binding to the Asialoglycoprotein Receptor (ASGR), a cell surface C-type lectin that functions as a scavenger receptor and is able to remove desialylated glycoproteins from circulation"
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ASGR1 homo-oligomers are sufficient for GalNAc-mediated endocytosis at the cell surface, whereas co-expression of ASGR1 incorporates ASGR2 into ASGR1/ASGR2 heterodimers that are exported from the ER to the plasma membrane.
"This data parallels previous reports demonstrating that co-expression of ASGR1 results in ASGR2 incorporation into ASGR1/ASGR2 heterodimers that are then exported from the ER and are ultimately targeted to the plasma membrane"
A reference map of the human binary protein interactome.
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High-throughput yeast two-hybrid interactome screen (HuRI) reporting binary protein interactions for human ORFs.
"Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'."
Determination of mouse major asialoglycoprotein receptor cDNA sequence.
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Hepatic asialoglycoprotein receptors specifically recognize galactose-terminated glycoproteins and mediate their receptor-mediated endocytosis.
"Hepatic lectins (asialoglycoprotein receptors) specifically recognize galactose-terminated glycoproteins and mediate endocytosis of these molecules."
PALM-C36-ASGR1:PALM-C54,58-ASGR2 binds desialated glycoproteins
UniProtKB record for human ASGR1 (P07306)
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ASGR1 is a hepatocyte transmembrane receptor that recognizes terminal galactose and N-acetylgalactosamine on desialylated plasma glycoproteins and mediates their endocytosis.
"Transmembrane protein predominantly expressed on hepatocytes that plays a key role in endocytosis of plasma glycoproteins that lack terminal sialic acid residues. Specifically recognizes terminal galactose and N-acetylgalactosamine residues, facilitating the clearance of desialylated glycoproteins from circulation."
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Isoform H1a is a single-pass type II plasma membrane protein.
"[Isoform H1a]: Cell membrane {ECO:0000269|PubMed:12171918}; Single-pass type II membrane protein."
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Calcium ions are required for ASGR1 carbohydrate ligand binding.
"Calcium is required for ligand binding."
Research and curation notes for human ASGR1