Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Panoramic view of a superfamily of phosphatases through substrate profiling.
UniProtKB reviewed entry for gmhB
QuickGO GOA annotations for gmhB
PANTHER family metadata for gmhB
Falcon (Edison Scientific) deep research report for gmhB (Q88RS0, PP_0059)
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"**GmhB** is defined as **D-glycero-β-D-manno-heptose 1,7-bisphosphate 7-phosphatase**, i.e., an enzyme that **removes the 7-phosphate from HBP** to produce **heptose-1-phosphate (HMP/HMP1)**."
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"This reaction is described as **dephosphorylation at the C-7 position** of HBP by GmhB."
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"Supports annotation of Q88RS0 as an HBP 7-phosphatase rather than a generic phosphatase; consistent with UniProt name for PP_0059."
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"GmhB occupies the third step of the conserved five-step ADP-heptose biosynthesis pathway supplying heptose for LPS inner-core assembly"
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"Thus, GmhB is best annotated as an enzyme of **LPS inner-core heptose donor biosynthesis**, rather than a signaling enzyme."
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"This strongly implies that GmhB acts in the **cytosolic** compartment where these metabolites are made and processed, with downstream glycosyltransfer steps contributing to envelope assembly."
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"Q88RS0 is best annotated as a **cytosolic HBP 7-phosphatase (GmhB)** that participates in **ADP-heptose donor biosynthesis**, enabling **LPS inner-core heptose incorporation** and thereby contributing to outer membrane/envelope integrity."
OpenScientist PSEPK ADP-heptose biosynthesis synthesis
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Genome-level analysis finds no credible HldD ortholog in KT2440, leaving the L,D-heptose endpoint unresolved while preserving the upstream ADP-D,D-heptose reactions.