Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Combined Automated Annotation using Multiple IEA Methods
TagR promotes PpkA-catalysed type VI secretion activation in Pseudomonas aeruginosa.
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PpkA autophosphorylation (Thr158/Thr161) and dimerisation of its extracellular domains activate the H1-T6SS through phosphorylation of Fha1; TagR acts upstream.
"We identified critical PpkA autophosphorylation sites and showed that small molecule-induced dimerization of the extracellular domains of PpkA is sufficient to activate the T6SS."
A novel serine/threonine protein kinase homologue of Pseudomonas aeruginosa is specifically inducible within the host infection site and is required for full virulence in neutropenic mice.
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ppkA is induced during infection and needed for full virulence in neutropenic mice; overexpression toxicity depends on an intact catalytic site.
"A ppkA null mutant strain shows reduced virulence in neutropenic mice compared to the wild type."
Threonine phosphorylation post-translationally regulates protein secretion in Pseudomonas aeruginosa.
UniProt record Q9I758 (ppkA)