Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
A novel lipolytic enzyme located in the outer membrane of Pseudomonas aeruginosa.
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EstA is an outer-membrane esterase with its catalytic domain exposed on the cell surface, active on short-chain p-nitrophenyl esters.
"Trypsin treatment of whole cells released a significant amount of esterase, indicating that the enzyme was located in the outer membrane with the catalytic domain exposed to the surface."
The autotransporter esterase EstA of Pseudomonas aeruginosa is required for rhamnolipid production, cell motility, and biofilm formation.
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estA deletion strongly reduces rhamnolipid production; motility and biofilm are affected; active-site Ser->Ala variant does not complement.
"None of the mutant phenotypes could be complemented by expression of EstA*, demonstrating that the phenotypes affected by the estA mutation depend on the enzymatically active protein."
Crystal structure of a full-length autotransporter.
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Full-length EstA structure; 12-stranded barrel, surface-exposed active site.
"The esterase active-site residues are located at the apical surface of the passenger"
Extracellular enzymes affect biofilm formation of mucoid Pseudomonas aeruginosa.
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estA overexpression in a mucoid strain enhances swimming and swarming, abolishes twitching and 3D biofilm, and raises rhamnolipid; effects may be indirect.
"Overexpression of estA resulted in a loss of twitching motility, although it enhanced the ability to swim and swarm."
Probing enzyme promiscuity of SGNH hydrolases.
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EstA functions as an esterase on p-nitrophenyl butyrate and lacks lipase and phospholipase activity in a 34-substrate panel.
"TesA, EstA and EstP were shown to function as esterases with different affinities and catalytic efficacies towards p-nitrophenyl butyrate"
Autotransporters with GDSL passenger domains: molecular physiology and biotechnological applications.
Enhanced rhamnolipid production by Pseudomonas aeruginosa overexpressing estA in a simple medium.
UniProtKB/Swiss-Prot entry O33407 (ESTA_PSEAE)