Identification of two gene clusters and a transcriptional regulator required for Pseudomonas aeruginosa glycine betaine catabolism.
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gbdR, a predicted AraC regulator, is required for growth on GB and DMG and for induction of the GB and DMG catabolic genes.
"Our screen also identified a predicted AraC"
GbdR regulates Pseudomonas aeruginosa plcH and pchP transcription in response to choline catabolites.
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GbdR mediates glycine betaine induction of plcH and pchP transcription.
"Here we report that the induction of plcH and pchP transcription by glycine betaine is mediated by GbdR, an AraC family transcription factor."
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MBP-GbdR binds the plcH and pchP promoters in EMSA.
"In electrophoretic mobility shift assays, a GbdR-maltose binding protein fusion bound specifically to both the plcH and pchP promoters."
Characterization of the GbdR regulon in Pseudomonas aeruginosa.
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GbdR activates 26 genes from 12 promoters, 11 bound by GbdR in vitro.
"Here we show that GbdR activation regulates transcription of 26 genes from 12 promoters, 11 of which have measureable binding to GbdR in vitro ."
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GbdR directly regulates the genes for every step from GB to pyruvate.
"as it directly regulates transcription of the genes involved in all steps of conversion from GB to pyruvate"
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods