Gene Ontology annotation based on Enzyme Commission mapping
Annotation inferences using phylogenetic trees
The phosphodiesterase DipA (PA5017) is essential for Pseudomonas aeruginosa biofilm dispersion.
-
Purified DipA has c-di-GMP phosphodiesterase activity and no diguanylate cyclase activity.
"Using biochemical and in vivo studies, we show that DipA associates with the membrane and exhibits phosphodiesterase activity but no detectable diguanylate cyclase activity."
-
Activity with c-di-GMP as substrate was measured directly, though low.
"Similar low activities were observed when c-di-GMP was used as a substrate"
-
The dipA mutant fails to disperse in response to several distinct cues, not glutamate alone.
"to be dispersion deficient in response to glutamate, nitric oxide, ammonium chloride, and mercury chloride."
UniProt entry Q9HUF2 (Q9HUF2_PSEAE)