Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification.
-
NDUFA11 is identified by mass spectrometry as a subunit of immunopurified human Complex I (NADH dehydrogenase).
"we can resolve and identify the human homologues of 42 polypeptides detected so far in the more extensively studied"
Accessory subunits are integral for assembly and function of human mitochondrial complex I.
-
Human Complex I comprises 45 subunits; 14 core subunits are shared with bacteria and 31 are accessory subunits whose incorporation increases energetic cost and requires assembly factors.
"Bacterial and human complex I share 14 core subunits that are essential for enzymatic function"
-
25 of the accessory subunits are strictly required for assembly of a functional Complex I, and loss of a subunit destabilizes co-module subunits.
"We show that 25 subunits are strictly required for assembly of a functional complex"
Architecture of Human Mitochondrial Respiratory Megacomplex I(2)III(2)IV(2).
-
Cryo-EM of the human respiratory megacomplex reveals the precise assignment of individual Complex I subunits, including NDUFA11.
"reveals the precise assignment of individual subunits of human CI"
Assembly of mammalian oxidative phosphorylation complexes I-V and supercomplexes.
-
OXPHOS complexes contain core catalytic proteins plus many supernumerary subunits with essential roles in assembly, regulation and stability; complexes I, III and IV form respiratory supercomplexes.
"'supernumerary' subunits that play essential roles in assembly, regulation and stability"
A reference map of the human binary protein interactome.
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
-
NDUFA11 is a member of the high-confidence human mitochondrial proteome (MitoCoP) defined by quantitative proteomics.
"mitochondrial high-confidence proteome of >1,100 proteins"
Complex I oxidises NADH to NAD+, reduces CoQ to CoQH2
Intermediate 1 binds HP subcomplex to form Intermediate 2
Peripheral arm subunits bind the 815kDa complex to form a 980kDa complex
Intermediate 2 binds MT-ND1:NDUFAF5:NDUFAF6 to form a 315kDa subcomplex
The MCIA complex, NDUFAF2-7 all dissociate from the 980kDa complex, resulting in Complex I
ND4, ND5 bind the 550kDa complex to form the 815kDa complex
The 315kDa subcomplex binds the 370kDa subcomplex to form the 550kDa complex