HBS1L PN Consistency Notes
- Generated: 2026-06-18
- Project: PROTEOSTASIS
- Scope: PN consistency rereview against local AIGR review and available deep-research artifacts
- UniProt: Q9Y450
- AIGR review status: COMPLETE
- Review batch: proteostasis-batch-2026-06-07c
- Batch change status: added
Source Files Checked
Deep Research Files
- No
*-deep-research*.md file found in this gene directory.
AIGR Review Snapshot
- Description: HBS1L (HBS1-like translational GTPase) is a cytoplasmic GTPase of the TRAFAC-class translation-factor superfamily, in the eEF1A/eRF3/Hbs1 group. It is the GTP-binding subunit of the Pelota-HBS1L complex (also called the Dom34-Hbs1 complex), partnering the eRF1-like factor PELO. The complex recognizes ribosomes stalled at the 3' end of an mRNA (truncated, non-stop, or no-go messages); HBS1L delivers PELO to the ribosomal A site and, through its GTPase activity, licenses PELO- and ABCE1-mediated splitting of the stalled 80S ribosome into subunits, thereby rescuing the ribosome and initiating no-go decay (NGD) and non-stop decay (NSD). Although phylogenetically related to the translation-termination factor eRF3, HBS1L does not possess eRF3 (peptide-release) activity. A short alternatively spliced isoform (HBS1LV3) instead scaffolds the cytoplasmic SKI complex and exosome via direct SKIC2 and EXOSC3 binding, coupling mRNA extraction to 3'-5' degradation.
- Existing/core annotation action counts: ACCEPT: 18; KEEP_AS_NON_CORE: 11; MARK_AS_OVER_ANNOTATED: 4
PN Consistency Summary
- Consistency: Fully consistent. Deep research, review, and PN all describe HBS1L as the GTPase subunit of the Pelota-HBS1L (Dom34-Hbs1) complex that licenses PELO/ABCE1 ribosome splitting and triggers no-go/non-stop decay. The review is rich and well-aligned (multiple IDA GO:0072344, GO:0032790 ribosome disassembly, GO:0070966 no-go decay, GO:1990533 Dom34-Hbs1 complex). The isoform-2 (HBS1LV3) SKI/exosome scaffolding role is an added reviewer dimension outside the PN frame, not a contradiction.
- PN story / NEW pressure: No NEW pressure. PN's rescue story (GO:0072344) is already in GOA exact and richly ACCEPTED in the review (IBA + 2×IDA + TAS). The group-level GO:0006515 is a broad parent already entailed by the specific rescue/RQC terms HBS1L carries. Conclusion: already captured (more specifically than the PN projection).
- Evidence alignment: PN lists no reference titles. Review cites the canonical mechanistic set: PMID:21448132 (Pelota/Hbs1/ABCE1 dissociation), PMID:27863242 (cryo-EM), PMID:23667253 (non-stop decay), PMID:20947765 (Dom34:Hbs1 yeast), PMID:9872408 (original eRF3-related characterization), PMID:28204585 (short-isoform SKI/exosome). No citation conflict; PN simply carries no PMIDs.
- Verdict: Fully consistent; PN rescue story already captured (more specifically) — no NEW pressure. Recommended edits: none warranted; treat group-node GO:0006515 as context-only/entailed for HBS1L (do not add as a separate annotation) [MAP].
Full Consistency Review
- UniProt: Q9Y450 · batch: proteostasis-batch-2026-06-07c · review status: COMPLETE
- PN placement:
Translation|Cytosolic translation|Ribosome-associated QC|Ribosomal rescue ; PN-node mapping: type Ribosomal rescue=mapped→GO:0072344 rescue of stalled cytosolic ribosome (already_in_goa_exact); group Ribosome-associated QC=mapped→GO:0006515 protein quality control (new_to_goa); class/branch=context_only.
- Consistency: Fully consistent. Deep research, review, and PN all describe HBS1L as the GTPase subunit of the Pelota-HBS1L (Dom34-Hbs1) complex that licenses PELO/ABCE1 ribosome splitting and triggers no-go/non-stop decay. The review is rich and well-aligned (multiple IDA GO:0072344, GO:0032790 ribosome disassembly, GO:0070966 no-go decay, GO:1990533 Dom34-Hbs1 complex). The isoform-2 (HBS1LV3) SKI/exosome scaffolding role is an added reviewer dimension outside the PN frame, not a contradiction.
- PN story / NEW pressure: No NEW pressure. PN's rescue story (GO:0072344) is already in GOA exact and richly ACCEPTED in the review (IBA + 2×IDA + TAS). The group-level GO:0006515 is a broad parent already entailed by the specific rescue/RQC terms HBS1L carries. Conclusion: already captured (more specifically than the PN projection).
- Mapping strategy: Mapping matches precedent correctly — type-node GO:0072344 is exact and already in GOA; group-node GO:0006515 is a broader parent and should be treated as entailed/context, not separately propagated (would duplicate the more specific rescue/no-go terms). No node change driven by this gene.
- Evidence alignment: PN lists no reference titles. Review cites the canonical mechanistic set: PMID:21448132 (Pelota/Hbs1/ABCE1 dissociation), PMID:27863242 (cryo-EM), PMID:23667253 (non-stop decay), PMID:20947765 (Dom34:Hbs1 yeast), PMID:9872408 (original eRF3-related characterization), PMID:28204585 (short-isoform SKI/exosome). No citation conflict; PN simply carries no PMIDs.
- Verdict: Fully consistent; PN rescue story already captured (more specifically) — no NEW pressure. Recommended edits: none warranted; treat group-node GO:0006515 as context-only/entailed for HBS1L (do not add as a separate annotation) [MAP].
PN Dossier Context
- review_batch: proteostasis-batch-2026-06-07c
- review_yaml: genes/human/HBS1L/HBS1L-ai-review.yaml
- PN workbook rows: 1
PN row 1: Translation | Cytosolic translation | Ribosome-associated QC | Ribosomal rescue
- UniProt: Q9Y450
- In branches: TR
- PN-node mapping records (path + ancestors):
- [type] Translation|Cytosolic translation|Ribosome-associated QC|Ribosomal rescue
status=mapped scope=ok_for_propagation_to_go GO=[GO:0072344 rescue of stalled cytosolic ribosome]
rationale: This PN RQC type denotes rescue of stalled cytosolic ribosomes. The matching GO process term is the direct target.
- [group] Translation|Cytosolic translation|Ribosome-associated QC
status=mapped scope=ok_for_propagation_to_go GO=[GO:0006515 protein quality control for misfolded or incompletely synthesized proteins]
rationale: The PN ribosome-associated quality-control group covers surveillance and disposal of stalled or defective nascent-chain translation products. GO lacks a dedicated ribosome-associated QC term in the local cache, so the broader protein-quality-control process is the best supported target.
- [class] Translation|Cytosolic translation
status=context_only scope=too_broad_to_propagate GO=[GO:0002181 cytoplasmic translation]
rationale: The PN class Cytosolic translation is centered on the cytoplasmic translation apparatus and process, but it also houses supporting machinery such as ribosome biogenesis factors. The GO process term is a useful high-level label for the class, but propagating it to all members would over-annotate genes whose PN placement is through assembly or maturation context rather than core cytoplasmic translation.
- [branch] Translation
status=context_only scope=too_broad_to_propagate GO=[GO:0006412 translation]
rationale: The PN Translation branch is organized around the translation apparatus and immediately associated cotranslational quality-control systems. GO translation is the closest high-level process label, but the PN branch also contains adjacent machinery such as ribosome biogenesis and nascent-chain handling. Keeping this relationship is useful for interpretation, but it is too broad to project safely onto every member.
Projected GO annotations (2)
- GO:0006515 protein quality control for misfolded or incompletely synthesized proteins | scope=ok_for_propagation_to_go | goa_status=new_to_goa | from=Translation|Cytosolic translation|Ribosome-associated QC
- GO:0072344 rescue of stalled cytosolic ribosome | scope=ok_for_propagation_to_go | goa_status=already_in_goa_exact | from=Translation|Cytosolic translation|Ribosome-associated QC|Ribosomal rescue
Note
This file is generated from the current PROTEOSTASIS phase-1 dossier and local gene-review artifacts. Edit the source review, PN mapping, or dossier rather than this generated note when correcting the underlying curation.