Human beta chains assemble with alpha and gamma chains into secreted fibrinogen. Chain-combination experiments in BHK recipients and the human fibrinogen structure establish a structural contribution by beta, beyond an indirect phenotype of gene loss (PMID:8910396(https://pubmed.ncbi.nlm.nih.gov/8910396/), assembly Results; PMID:19296670(https://pubmed.ncbi.nlm.nih.gov/19296670/), Overall Structure). The single structural core includes clot formation. Thrombin releases fibrinopeptide B; other proteins perform proteolytic catalysis and cross-linking.
Whole-complex cell adhesion and platelet aggregation remain non-core contextual effects. The positive inherited PAINT assertions are retained with their actual node and sequence evidence; they are not rejected because the target appears among its own descendant evidence. The isolated modified beta-chain preparation was inactive in the platelet assay, and that result is not generalized to all assembled beta-chain states (PMID:6281794(https://pubmed.ncbi.nlm.nih.gov/6281794/), Table 1). Alpha-C cofactor experiments do not establish a beta-chain enzyme-substrate adaptor core.
The mouse-donor cortex assertion is refined to the external side of the plasma membrane. The recovered experiment measures added fluorescent fibrinogen at the outside of mouse megakaryocytes, with unspecified ligand species; separate human platelet evidence identifies surface-associated fibrin beta chains (PMID:15914557(https://pubmed.ncbi.nlm.nih.gov/15914557/), Methods and Figure 1; PMID:6777381(https://pubmed.ncbi.nlm.nih.gov/6777381/), Figure 3). The human target is not assigned a mouse recipient identity or an intracellular cortex location from peripheral fluorescence.
All 113 decisions and all 82 reference assessments are integrated. The 65 ACCEPT, 28 KEEP_AS_NON_CORE, five TOO_GENERAL, one MODIFY and 14 UNDECIDED decisions preserve source-specific uncertainty where original experiments or exact target records remain inaccessible. No new GO annotation or publication quotation is introduced. The direct journal, direct evidence guide and database evidence guide retain precise evidence scopes and access limits.
Two cleavage-product records describe official precursor coordinates separately from every imported parent-accession annotation. Proteomic accession groups do not prove a natural splice or released peptide. The genuine configured research-provider invocation produced no report; private traces were not read to infer a cause, and these notes document manual evidence assessment.
Align the core process with GO:0072378 blood coagulation, fibrin clot formation, already accepted in the review and supported by the structural assembly evidence. This does not imply that FGB performs the proteolysis or cross-linking catalysed by other proteins. Clarify the four HDA UNDECIDED reasons: independent gene-level extracellular/structural support does not recover an unverified source-specific protein/sample identification. The alpha/gamma variant paper and colostrum survey receive MEDIUM relevance with their access and target limits preserved. Supported generic binding remains non-core under the campaign instruction. No NEW annotation, new quotation, product change or source-cache edit is introduced.