Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
PANTHER PTHR24198 PAINT annotations
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PTN000917496 carries the current fungal APSES-family IBD assertions for the SWI4 IBA rows.
"PTHR24198 PTN000917496 GO:0005737 C IBD false SGD:S000004172 taxon:33154 20260224
PTHR24198 PTN000917496 GO:0030907 C IBD false PomBase:SPAC22F3.09c|PomBase:SPBC336.12c|PomBase:SPBC725.16|SGD:S000002214|SGD:S000004172 taxon:33154 20181004
PTHR24198 PTN000917496 GO:0000978 F IBD false PomBase:SPAC22F3.09c|PomBase:SPBC336.12c|PomBase:SPBC725.16 taxon:33154 20260224
PTHR24198 PTN000917496 GO:0001228 F IBD false PomBase:SPAC22F3.09c|SGD:S000000913|SGD:S000002214 taxon:33154 20240418
PTHR24198 PTN000917496 GO:0000082 P IBD false CGD:CAL0000174900|CGD:CAL0000183437|CGD:CAL0000186541|SGD:S000000913|SGD:S000002214|SGD:S000004172 taxon:33154 20260224
PTHR24198 PTN000917496 GO:0045944 P IBD false CGD:CAL0000174900|CGD:CAL0000183437|CGD:CAL0000186541|PomBase:SPAC22F3.09c|PomBase:SPBC336.12c|PomBase:SPBC725.16|SGD:S000000913|SGD:S000002214|SGD:S000004172|UniProtKB:Q5B8H6 taxon:33154 20240418"
Swi4-dependent SWI4 transcription couples cell size to cell cycle commitment.
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Swi4-dependent SWI4 autoregulation contributes to Swi4 accumulation during G1 and to the timing of Start.
"The role of Swi4-dependent SWI4 transcription in the Start transition is supported by four main lines of evidence"
Regulation of cell cycle transcription factor Swi4 through auto-inhibition of DNA binding.
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Swi4 remains nuclear throughout the cell cycle, in contrast to Swi6.
"We conclude that, unlike Swi6, whose localization changes throughout the cell cycle, Swi4 remains nuclear throughout the cell cycle."
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Full-length Swi4 cannot bind SCBs without Swi6 because its C terminus intramolecularly masks the N-terminal DNA-binding domain; Swi6 binding to the C terminus relieves this.
"The interaction of the carboxy-terminal region of Swi4 with Swi6 alleviates this inhibition, allowing Swi4 to bind DNA."
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Swi4 is monomeric in solution and SBF is a 1:1 Swi4-Swi6 heterodimer.
"SBF ran at 180 kDa, a size which is close to the predicted size of a heterodimer of Swi4 (123 kDa) and Swi6 (91 kDa)."
Complex transcriptional circuitry at the G1/S transition in Saccharomyces cerevisiae.
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Swi4 binds ~183 promoters by ChIP-chip, including CLN2, its own promoter and a set of transcription-factor genes, confirmed by ChIP-PCR.
"Twenty-two of the 26 promoters tested were enriched in Swi4–HA immunoprecipitates over immunoprecipitated DNA from the untagged strain."
Interaction of the yeast Swi4 and Swi6 cell cycle regulatory proteins in vitro.
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Swi4 and Swi6 associate directly through C-terminal regions; Swi4 recognises the SCB sequence without Swi6.
"we propose that Swi4 is responsible for binding to the SCB sequence while Swi6, through its association with Swi4, regulates activity of the complex."
Proteome survey reveals modularity of the yeast cell machinery.
Yeast Mpk1 mitogen-activated protein kinase activates transcription through Swi4/Swi6 by a noncatalytic mechanism that requires upstream signal.
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Cell-wall-stress activation of FKS2 requires SBF and activated Mpk1 but not Mpk1 catalytic activity; Mpk1 and Swi4 bind the FKS2 promoter codependently and independently of Swi6, which is then recruited.
"Promoter association of Mpk1 and the Swi4 DNA-binding subunit of SBF were codependent but did not require Swi6, indicating that the MAPK confers DNA-binding ability to Swi4."
The role of SWI4 and SWI6 in the activity of G1 cyclins in yeast.
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The essential, overlapping role of SWI4 and SWI6 is to ensure transcription of the G1 cyclin genes CLN1 and CLN2.
"We show that the essential role of SWI4 and SWI6 is to ensure the activity of G1-specific cyclin genes."
A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters.
Transcriptional reporters for genes activated by cell wall stress through a non-catalytic mechanism involving Mpk1 and SBF.
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Induction of FKS2, CHA1, YKR013w and YLR042c reporters by cell-wall stress (39 degrees C, Congo Red, calcofluor white, Zymolyase) is strictly SWI4- and SWI6-dependent.
"Transcriptional induction of all of these reporters by cell wall stress was strictly dependent on both SWI4 and SWI6"
Defining the budding yeast chromatin-associated interactome.
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mChIP-MS of Swi4-TAP recovers the SBF partner Swi6 and chromatin-associated regulators such as Stb1.
"mChIP-MS analyses of Swi4-TAP, Swi6-TAP and Mbp1-TAP successfully identified known interaction partners (such as Stb1) for both MBF and SBF"
3,6-Epidioxy-1,10-bisaboladiene inhibits G1 -specific transcription through Swi4/Swi6 and Mbp1/Swi6 via the Hog1 stress pathway in yeast.
Identification of a DNA binding factor involved in cell-cycle control of the yeast HO gene.
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SWI4 and SWI6 are required for formation of the cell-cycle-box factor (CCBF) complex in vitro and are the only SWI genes required for CCB-driven expression in vivo.
"We also show that SWI4 and SWI6 are the only SWI genes required for expression in vivo from the CCB sequences."
The yeast SWI4 protein contains a motif present in developmental regulators and is part of a complex involved in cell-cycle-dependent transcription.
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Anti-Swi4 antibodies show that Swi4 protein is present in the CCBF-DNA complex; SWI4 contains two copies of the Swi6/cdc10 (ankyrin) motif.
"We demonstrate by using antibodies to the SWI4 protein in gel-shift assays that the protein is present in the CCBF-DNA complex."
Cell cycle control of the yeast HO gene: cis- and trans-acting regulators.
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SWI4 and SWI6 are specifically required for activation of transcription through the CACGA4 (SCB) element of the HO promoter.
"SWI4 and SWI6 are specifically required for CACGA4-mediated activation of transcription."
The social and structural architecture of the yeast protein interactome.
Analysis of the SWI4/SWI6 protein complex, which directs G1/S-specific transcription in Saccharomyces cerevisiae.
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The sequence-specific DNA-binding domain resides in Swi4; the Swi4 C terminus is necessary and sufficient for Swi6 association; Swi6 controls access of the Swi4 DNA-binding domain to DNA.
"From these data, we propose that the sequence-specific DNA-binding domain resides in SWI4 but that SWI6 controls the accessibility of this domain in the SWI4/6 complex."
SWI4 deep research (falcon literature synthesis)
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Swi4 is constitutively nuclear; regulation of SBF is by auto-inhibition, Swi6, Whi5 and cyclin-Cdk1 rather than by Swi4 nuclear export.
"Swi4 is predominantly **nuclear** and was reported to remain nuclear throughout the mitotic cell cycle."
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SBF is Swi4-Swi6; MBF is the paralogous Mbp1-Swi6 complex and does not contain Swi4.
"SBF contains Swi4 plus Swi6, whereas the related MBF complex substitutes Mbp1 for Swi4 while retaining Swi6."
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The Swi4 C-to-N-terminal contact is an intramolecular auto-inhibitory fold-back, not homo-oligomerisation.
"Evidence that full-length Swi4 is monomeric favors **intramolecular** masking rather than inhibition through Swi4 oligomerization."
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The central ankyrin repeats of Swi4 bind the mitotic Clb2-Cdc28 kinase (Siegmund and Nasmyth 1996, not cached here).
"Four ankyrin repeats mediate protein interactions, including association with mitotic **Clb2–Cdc28/Cdk1**."
Exploring the yeast acetylome using functional genomics.
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Swi4 is acetylated at K1016 and K1066 in its Swi6-binding C-terminal domain.
"Using mass spectrometry, we identified two acetylation sites, K1016 and K1066 (Figure S3), in the C-terminal domain of Swi4, which is required for interaction with its regulatory partner, Swi6"
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A deacetylation-mimic Swi4 (both lysines to arginine) is defective in CLN2 induction and in stable association with Swi6 at G1 promoters; Rpd3 loss raises Swi4 acetylation.
"suggesting that acetylation of Swi4 may be needed for a stable association with Swi6 at G1 promoters."
SGD gene description for SWI4 (yeast)