Gene Ontology annotation through association of InterPro records with GO terms
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
TreeGrafter-generated GO annotations
UniProtKB entry for PSEPK gatC
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GatC is the small structural subunit of heterotrimeric GatABC.
"SUBUNIT: Heterotrimer of A, B and C subunits."
Direct glutaminyl-tRNA biosynthesis and indirect asparaginyl-tRNA biosynthesis in Pseudomonas aeruginosa PAO1.
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Native PAO1 uses direct GlnRS and indirect Asn formation; its GatABC also transamidates supplied heterologous Glu-tRNA(Gln) in vitro. Do not infer native KT2440 Gln-transamidation flux from that capacity.
"The heterotrimeric AdT of P. aeruginosa has no Glu-tRNA Gln substrate in its host, but it can transamidate heterologous Glu-tRNA Gln ."
Structure of the Pseudomonas aeruginosa transamidosome reveals unique aspects of bacterial tRNA-dependent asparagine biosynthesis.
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PAO1 transamidosome structure and kinetics distinguish stabilization of the mischarged Asp-tRNA substrate from product release. This is a comparative architectural basis, not a native KT2440 complex or error-rate measurement.
"However, Pa GatCAB does protect Asp-tRNA Asn from deacylation"
Forward Genetics Reveals a gatC-gatA Fusion Polypeptide Causes Mistranslation and Rifampicin Tolerance in Mycobacterium smegmatis.
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Native M. smegmatis GatC mutations and a GatC-GatA fusion alter mistranslation. This supports conserved scaffold involvement in fidelity, not a KT2440 error-rate or antibiotic-tolerance experiment.
"Our results suggest that mutations in all three constituent members of gatCAB may result in adaptive mistranslation"
Clinically Relevant Mutations of Mycobacterial GatCAB Inform Regulation of Translational Fidelity.
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Mycobacterial GatCAB/transamidosome stabilization affects translational errors. The assays distinguish a complex-stability mechanism from amino-acid activation; transfer to KT2440 is mechanistic inference.
"trehalose supplementation of cultures of mycobacterial strains expressing wild-type GatCAB caused a reduction in translational errors specifically for the indirect tRNA aminoacylation pathway."