Gene Ontology annotation through association of InterPro records with GO terms
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
UniProtKB record B9U5L8 (B9U5L8_VARPD) - 3MP dioxygenase Mdo from Variovorax paradoxus TBEA6
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VPARA_05600 (mdo, B9U5L8; MetaCyc MONOMER-18546, BRENDA 1.13.11.91) is a 195-aa cupin-fold thiol dioxygenase (CDD cd10548, Pfam PF05995, InterPro IPR010300) with a catalytic Fe(2+)-binding 3-His triad at His90, His92, and His143.
"DR BioCyc; MetaCyc:MONOMER-18546; -.
DR BRENDA; 1.13.11.91; 234."
3-mercaptopropionate dioxygenase, a cysteine dioxygenase homologue, catalyzes the initial step of 3-mercaptopropionate catabolism in the 3,3-thiodipropionic acid-degrading bacterium variovorax paradoxus.
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Purified Variovorax paradoxus TBEA6 Mdo (B9U5L8) catalyzes the oxygenation of 3-mercaptopropionic acid to 3-sulfinopropionate in vivo and in vitro and does not utilize L-cysteine or cysteamine.
"Detection of 3-sulfinopropionate in the supernatant of one of these mutants during cultivation on 3,3-thiodipropionic acid as well as in vivo and in vitro enzyme assays using purified protein demonstrated oxygenation of 3-mercaptopropionic acid to 3-sulfinopropionate by this enzyme; cysteine and cysteamine were not used as substrate."